TFAM, 43-246aa, Human, His tag, E Coli | MyBioSource MBS203770 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TFAM, 43-246aa, Human, His tag, E Coli

catalog :

MBS203770

quantity :

0.01 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203770

products type :

Recombinant Protein

products full name :

TFAM, 43-246aa, Human, His tag, E Coli

products short name :

TFAM

products name syn :

Transcription factor A; mitochondrial precursor; MtTF1; mtTFA; TCF6; TCF6L1; TCF6L2; TCF6L3

other names :

transcription factor A, mitochondrial isoform 1; Transcription factor A, mitochondrial; transcription factor A, mitochondrial; mitochondrial transcription factor 1; mitochondrial transcription factor A; transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; transcription factor A, mitochondrial; Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2

products gene name :

TFAM

other gene names :

TFAM; TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3; TCF6; TCF6L2; mtTFA; MtTF1; TCF-6

uniprot entry name :

TFAM_HUMAN

sequence length :

246

sequence :

MGSSHHHHHH SSGLVPRGSH MSSVLASCPK KPVSSYLRFS KEQLPIFKAQ NPDAKTTELI RRIAQRWREL PDSKKKIYQD AYRAEWQVYK EEISRFKEQL TPSQIMSLEK EIMDKHLKRK AMTKKKELTL LGKPKRPRSA YNVYVAERFQ EAKGDSPQEK LKTVKENWKN LSDSEKELYI QHAKEDETRY HNEMKSWEEQ MIEVGRKDLL RRTIKKQRKY GAEEC

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Transcription & Translation

products description :

TFAM, also known as MtTF1 or mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. TFAM is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. TFAM has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting. Recombinant human TFAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Sinha RA., et al. (2010) Biochem Biophys Res Commun. 397(3):548-52. Aquilano K., et al. (2010) J Biol Chem. 285(28):21590-9.

ncbi gi num :

4507401

ncbi acc num :

NP_003192

ncbi gb acc num :

NM_003201.2

ncbi mol weight :

26.6 kDa (225aa), confirmed by MALDI-TOF

ncbi pathways :

Energy Metabolism Pathway (198907); Gene Expression Pathway (105937); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Mitochondrial Gene Expression Pathway (198757); Mitochondrial Biogenesis Pathway (1127495); Mitochondrial Transcription Initiation Pathway (106569); Organelle Biogenesis And Maintenance Pathway (1127494); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948); Transcription From Mitochondrial Promoters Pathway (106568)

ncbi summary :

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

uniprot summary :

TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M. Protein type: Transcription regulation; DNA-binding. Chromosomal Location of Human Ortholog: 10q21. Cellular Component: mitochondrion; mitochondrial matrix; cytosol; nucleus. Molecular Function: protein binding; heat shock protein binding; chromatin binding; transcription factor activity; DNA bending activity. Biological Process: mitochondrial respiratory chain complex assembly; chromatin remodeling; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; gene expression; positive regulation of transcription from RNA polymerase II promoter; DNA-dependent DNA replication; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter

size1 :

0.01 mg

price1 :

310 USD

size2 :

0.05 mg

price2 :

720

more info or order :

MyBioSource product webpage