protein

DDAH1, 1-285aa, Human, His tag, E Coli

MBS203757

0.1 mg

310 USD

Recombinant Protein

DDAH1, 1-285aa, Human, His tag, E Coli

DDAH1

Dimethylarginine dimethylaminohydrolase 1; DDAH; DDAHI; Dimethylargininase-1

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH-1; DDAHI; NG, NG-dimethylarginine dimethylaminohydrolase; dimethylargininase-1; epididymis secretory protein Li 16; dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1

DDAH1

DDAH1; DDAH1; DDAH; HEL-S-16; DDAH; DDAH-1; Dimethylarginine dimethylaminohydrolase 1

DDAH1_HUMAN

285

MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Neuroscience

Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation. Recombinant human DDAH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

NaKagomi S., et al. (1999) Eur J Neurosci. 11:2160-2166. Leiper J., et al. (2002) J Biol Chem. 276:40449-40456.

6912328

NP_036269

NM_012137.3

33.5 kDa (308aa) confirmed by MALDI-TOF

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]

DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.5.3.18; Hydrolase. Chromosomal Location of Human Ortholog: 1p22. Cellular Component: mitochondrion. Molecular Function: amino acid binding; dimethylargininase activity; metal ion binding; catalytic activity. Biological Process: positive regulation of angiogenesis; positive regulation of nitric oxide biosynthetic process; citrulline metabolic process; regulation of systemic arterial blood pressure; arginine catabolic process; nitric oxide mediated signal transduction

0.1 mg

310 USD

0.5 mg

720