catalog number :
MBS203743
products type :
Recombinant Protein
products full name :
HCV NS3, 1225-1456aa, HCV, His tag, E Coli
products short name :
HCV NS3
products name syn :
NS3 protease/helicase HCV NS3 transactivated protein; NS 3; NS3P; P70; Serine protease/NTPase/helicase.
other names :
polyprotein; polyprotein; core protein; E1 protein; E2 protein; p7 protein; NS2 protein; NS3 protease/helicase; NS4A protein; NS4B protein; NS5A protein; NS5B RNA-dependent RNA polymerase
products gene name :
HCV NS3
other gene names :
HCVgp1
sequence :
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT
purity :
> 95% by SDS-PAGE
form :
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: HCV. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Virus
products description :
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Hahn JA., et al. (2007) J Infect Dis. 195(11):1556-9. Caruntu FA., et al. (2006) J Gastrointestin Live Dis. 15(3):249-56.
ncbi gb acc num :
NC_004102.1
ncbi mol weight :
27.9 kDa (268aa), confirmed by MALDI-TOF.