protein

ACAA1, 27-424aa, Human, His tag, E Coli

MBS203719

0.1 mg

310 USD

Recombinant Protein

ACAA1, 27-424aa, Human, His tag, E Coli

ACAA1

ACAA; PTHIO; THIO; 3-ketoacyl-CoA thiolase; peroxisomal

3-ketoacyl-CoA thiolase, peroxisomal isoform a; 3-ketoacyl-CoA thiolase, peroxisomal; 3-ketoacyl-CoA thiolase, peroxisomal; acetyl-Coenzyme A acyltransferase 1; beta-ketothiolase; peroxisomal 3-oxoacyl-CoA thiolase; peroxisomal 3-oxoacyl-Coenzyme A thiolase; acetyl-CoA acyltransferase 1; Acetyl-CoA acyltransferase; Beta-ketothiolase; Peroxisomal 3-oxoacyl-CoA thiolase

ACAA1

ACAA1; ACAA1; ACAA; THIO; PTHIO; ACAA; PTHIO

THIK_HUMAN

424

MGSSHHHHHH SSGLVPRGSH MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN

> 95 % by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Enzymes & Proteases

ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Recombinant human ACAA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Bout A., et al. (1991) Biochim Biophys Acta. 1090(1):43-51. Patel S., et al. (2003) Eur Respir J. 22(5):755-60.

4501853

NP_001598

NM_001607.3

43.8 kDa (419aa) confirmed by MALDI-TOF

Beta-oxidation Of Very Long Chain Fatty Acids Pathway (106139); Biosynthesis Of Unsaturated Fatty Acids Pathway (83034); Biosynthesis Of Unsaturated Fatty Acids Pathway (429); Fatty Acid Degradation Pathway (82935); Fatty Acid Degradation Pathway (296); Fatty Acid Metabolism Pathway (868084); Fatty Acid Metabolism Pathway (878045); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

ACAA1: an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]. Protein type: EC 2.3.1.16; Acetyltransferase; Lipid Metabolism - unsaturated fatty acid biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lipid Metabolism - fatty acid. Chromosomal Location of Human Ortholog: 3p22.2. Cellular Component: peroxisomal matrix; intracellular membrane-bound organelle; membrane; peroxisome. Molecular Function: palmitoyl-CoA oxidase activity; protein binding; acetyl-CoA C-acyltransferase activity. Biological Process: fatty acid beta-oxidation using acyl-CoA oxidase; fatty acid beta-oxidation; very-long-chain fatty acid metabolic process; bile acid metabolic process; unsaturated fatty acid metabolic process; cellular lipid metabolic process. Disease: D-bifunctional Protein Deficiency

0.1 mg

310 USD

0.5 mg

720