protein

SOD2, 25-222aa, Human, His tag, E Coli

MBS203695

0.1 mg

285 USD

Recombinant Protein

SOD2, 25-222aa, Human, His tag, E Coli

SOD2

IPOB; MNSOD; MVCD6; Superoxide dismutase [Mn]; mitochondrial IPO B; Manganese SOD; Manganese superoxide dismutase; Mn SOD; SOD 2; SOD2; Superoxide dismutase [Mn] mitochondrial; Superoxide dismutase 2 mitochondrial; Superoxide Dismutase-2

superoxide dismutase; Superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; Mn superoxide dismutase; indophenoloxidase B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; superoxide dismutase 2, mitochondrial

SOD2

SOD2; SOD2; IPOB; MNSOD; MVCD6

SODM_HUMAN

222

MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK

> 95% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume. Assay: 1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochromC, 0.05mM xanthine, 0.005 units xanthine oxidase. 2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer. 3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

Redox Proteins

SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human SOD2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

MacMillan-Crow L.A., et al. (1999) Arch. Biochem. Biophys. 366:82-88. Prunotto M, et al. (2010) J Am Soc Nephrol. 21(3):507-19.

67782307

NP_001019636

NM_001024465.1

24.4kDa (219aa), confirmed by MALDI-TOF.

Cellular Responses To Stress Pathway (645258); DNA Damage Response (only ATM Dependent) Pathway (198827); Detoxification Of Reactive Oxygen Species Pathway (1127552); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Oxidative Stress Pathway (198916); Peroxisome Pathway (131226); Peroxisome Pathway (131126)

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Mitochondrial; EC 1.15.1.1. Chromosomal Location of Human Ortholog: 6q25.3. Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane. Molecular Function: identical protein binding; DNA binding; manganese ion binding; superoxide dismutase activity; oxygen binding. Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; response to lipopolysaccharide; locomotory behavior; response to L-ascorbic acid; protein homotetramerization; post-embryonic development; negative regulation of cell proliferation; response to selenium ion; glutathione metabolic process; regulation of mitochondrial membrane potential; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; negative regulation of neuron apoptosis; response to axon injury; response to electrical stimulus; response to drug; erythrophore differentiation; response to superoxide; release of cytochrome c from mitochondria; superoxide metabolic process; negative regulation of fat cell differentiation; liver development; regulation of transcription from RNA polymerase II promoter; response to manganese ion; iron ion homeostasis; response to reactive oxygen species; response to silicon dioxide; response to hyperoxia; response to cadmium ion; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; age-dependent response to reactive oxygen species; detection of oxygen; negative regulation of fibroblast proliferation; response to zinc ion; response to hypoxia; neuron development; response to activity; response to cold; superoxide release; induction of apoptosis by oxidative stress; hydrogen peroxide biosynthetic process. Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6

0.1 mg

285 USD

0.5 mg

640