WARS, 1-471aa, Human, His tag, E Coli | MyBioSource MBS203690 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

WARS, 1-471aa, Human, His tag, E Coli

catalog :

MBS203690

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203690

products type :

Recombinant Protein

products full name :

WARS, 1-471aa, Human, His tag, E Coli

products short name :

WARS

products name syn :

GAMMA-2; IFI53; IFP53; Tryptophanyl-tRNA synthetase; cytoplasmic; TrpRS; hWRS

other names :

tryptophan--tRNA ligase, cytoplasmic isoform a; Tryptophan--tRNA ligase, cytoplasmic; tryptophan--tRNA ligase, cytoplasmic; hWRS; interferon-induced protein 53; trpRS; tryptophan tRNA ligase 1, cytoplasmic; tryptophanyl-tRNA synthetase; Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS

products gene name :

WARS

other gene names :

WARS; WARS; IFI53; IFP53; GAMMA-2; IFI53; WRS; IFP53; TrpRS; hWRS

uniprot entry name :

SYWC_HUMAN

sequence length :

471

sequence :

MGSSHHHHHH SSGLVPRGSH MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Angiogenesis

products description :

WARS, also known as tryptophanyl-tRNA synthetase, belongs to the class I tRNA synthetase family. Two forms of tryptophanyl tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. It also regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Recombinant human WARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Nagano K., et al. (2004) Oncogene. 23(9):1693-703.

ncbi gi num :

47419916

ncbi acc num :

NP_776049

ncbi gb acc num :

NM_173701.1

ncbi mol weight :

55.3 kDa (491aa), confirmed by MALDI-TOF

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); Tryptophan Metabolism Pathway (198850); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - tryptophan; Ligase; EC 6.1.1.2; Translation. Chromosomal Location of Human Ortholog: 14q32.31. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: protein binding; tryptophan-tRNA ligase activity; ATP binding. Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation; translation; gene expression; angiogenesis; regulation of angiogenesis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage