protein

Alpha-enolase, 1-434aa, Human, Recombinant, E.Coli

MBS203689

0.1 mg

285 USD

Recombinant Protein

Alpha-enolase, 1-434aa, Human, Recombinant, E.Coli

Alpha-enolase

Non-Neuronal Enolase (NNE), Enolase-1(ENO1), MBP-1, PPH, tau-crystallin

alpha-enolase isoform 1; Alpha-enolase; alpha-enolase; c-myc promoter-binding protein-1; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase-alpha; non-neural enolase; phosphopyruvate hydratase; plasminogen-binding protein; tau-crystallin; enolase 1, (alpha); 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

ENO1; ENO1; NNE; PPH; MPB1; ENO1L1; ENO1L1; MBPB1; MPB1; NNE

ENOA_HUMAN

434

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM MgSO4, 10% Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at +4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method)

Expression System: E Coli. Bioactivity: Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C. Activity Assay: 1. Prepare 180ul assay buffer into a suitable container: The concentrations are 80 mM Triethanolamine (pH 6.5), 0.3 mM 2-phosphorglycerate, 0.2 mM beta- NADH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.2 mM ADP, 3.5 unit pyruvate kinase, 5 unit L-lactic dehydrogenas. 2. Equilibrate to 37C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 20ul of recombinant a-enolase protein 10ug/ml in assay buffer. 4. Record the decrease in A340nm for 5 minutes at 37C.

Metabolism

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E.coli and purified by using conventional chromatography.

Das R., et al. (2009) Blood. 113(22):5371-2. ueno NT., et al. (2008) Cancer Res. 68(22):9302-10.

4503571

NP_001419

NM_001428.3

47.1 kDa (434aa), confirmed by MALDI-TOF

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (106199); Glycogen Storage Diseases Pathway (1127581)

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene. Protein type: Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transcription, coactivator/corepressor; EC 4.2.1.11. Chromosomal Location of Human Ortholog: 1p36.2. Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane; M band; cytosol; nucleus. Molecular Function: protein binding; DNA binding; magnesium ion binding; phosphopyruvate hydratase activity; transcription corepressor activity; transcription factor activity. Biological Process: glycolysis; transcription, DNA-dependent; response to virus; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; gluconeogenesis

0.1 mg

285 USD

0.5 mg

640