catalog number :
MBS203674
products type :
Recombinant Protein
products full name :
uDP, 1-253aa, E Coli, His tag, E Coli
products short name :
uDP
products name syn :
urdPase; upase; uridine phosphorylase
other names :
uridine phosphorylase; Uridine phosphorylase; uridine phosphorylase
other gene names :
udp; udp; ECK3825; JW3808; UPase; UrdPase
uniprot entry name :
UDP_ECOLI
sequence :
MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL
purity :
> 95% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Enzymes & Proteases
products description :
uridine phosphorypase(uDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, urdPase, uPH, and uPase. Recombinant E.coli uDP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Burling F.T., et al. (2003) Acta Crystallogr. 59:73-76. Walton L., et al (1989) Nucleic Acids Res. 17(16):6741.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
29.3kDa (273aa) confirmed by MALDI-TOF
ncbi pathways :
Metabolic Pathways 131985!!Pyrimidine Metabolism Pathway 1023!!Pyrimidine Metabolism Pathway 309!!Pyrimidine Ribonucleosides Degradation Pathway 213!!Pyrimidine Ribonucleosides Degradation Pathway 139587!!Salvage Pathways Of Pyrimidine Ribonucleotides 120
ncbi summary :
Binds TrxA (Kumar, 2004). [More information is available at EcoGene: EG11045].