ARG1, 1-322aa, Human, His tag, E Coli | MyBioSource MBS203664 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

ARG1, 1-322aa, Human, His tag, E Coli

catalog :

MBS203664

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203664

products type :

Recombinant Protein

products full name :

ARG1, 1-322aa, Human, His tag, E Coli

products short name :

ARG1

products name syn :

Arginase-1; liver; Arginase-1; liver A I; Al; ARG 1; ARG1; Arginase 1; Arginase liver; Arginase type I; Arginase1; Liver type arginase; Type I arginase.

other names :

arginase-1 isoform 2; Arginase-1; arginase-1; arginase, liver; liver-type arginase; type I arginase; arginase 1; Liver-type arginase; Type I arginase

products gene name :

ARG1

other gene names :

ARG1; ARG1

uniprot entry name :

ARGI1_HUMAN

sequence length :

322

sequence :

MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH

purity :

> 85% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl

concentration :

0.5mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. Recombinant human ARG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Wu G., et al (1998) The Biochemical journal 336 (Pt 1): 1-17. Iyer RK., et al (2002) Molecular and cellular biology 22 (13): 4491-8.

ncbi gi num :

10947139

ncbi acc num :

NP_000036

ncbi gb acc num :

NM_000045.3

ncbi mol weight :

35.8kDa (330aa), confirmed by MALDI-TOF

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); IL4-mediated Signaling Events Pathway (137933); Metabolic Pathways (132956); Metabolism Pathway (477135)

ncbi summary :

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; Amino Acid Metabolism - arginine and proline; EC 3.5.3.1. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus. Molecular Function: manganese ion binding; arginase activity. Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; liver development; response to amino acid stimulus; response to vitamin A; response to manganese ion; response to vitamin E; response to selenium ion; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; lung development; urea cycle; response to amine stimulus. Disease: Argininemia

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage