CDA, 1-146aa, Human, His tag, E Coli | MyBioSource MBS203660 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

CDA, 1-146aa, Human, His tag, E Coli

catalog :

MBS203660

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203660

products type :

Recombinant Protein

products full name :

CDA, 1-146aa, Human, His tag, E Coli

products short name :

CDA

products name syn :

CDD; Cytidine aminohydrolase; Cytosine nucleoside deaminase; Cytidine deaminase

other names :

Cytidine deaminase; Cytidine deaminase; cytidine deaminase; cytidine aminohydrolase; cytosine nucleoside deaminase; small cytidine deaminase; cytidine deaminase; Cytidine aminohydrolase

products gene name :

CDA

other gene names :

CDA; CDA; CDD; CDD

uniprot entry name :

CDD_HUMAN

sequence length :

146

sequence :

MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM DTT, 2 mM EDTA, 100 mM NaCl, 40% Glycerol

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity:> 3.5 unit/mg. One unit of activity was defined as the amount required to deaminate 1.0 umole of cytidine per min at pH 7.5 at 25C. Assay: 1. Prepare 150 ul assay buffer. The final concentrations are 50 mM Tris-HCl (pH 7.5), 100 uM cytidine. 2. Add 50 ul recombinant CDA protein with concentration 0.25 ug in assay buffer. 3. Mix by inversion and records the decrease at A280nm for approximately 5 minutes. Cytidine millimolar extinction coefficient:. 5.27 (mM-1.cm-1). 100 uM / 50 mM Tris-HCl (pH 7.5) . PerkinElmer VICTOR X3 / A280nm. uV Plate (Costar, Cat. No. 3635). Cytidine (Sigma, Cat. No. C4654)

products categories :

Enzymes & Proteases

products description :

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Evrard A., et al. (2010) Ther Drug Monit. 32(1):53-60. Seeberg EC., et a/. (1998) Blood. 91(11):4127-35.

ncbi gi num :

32450432

ncbi acc num :

AAH54036

ncbi mol weight :

18.3 kDa(166aa), confirmed by MALDI-TOF

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Pyrimidine Salvage Reactions Pathway (106284)

ncbi summary :

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

uniprot summary :

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2 -deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family. Protein type: EC 3.5.4.5; Nucleotide Metabolism - pyrimidine; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes. Chromosomal Location of Human Ortholog: 1p36.2-p35. Cellular Component: extracellular region; cytosol. Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity. Biological Process: cell surface receptor linked signal transduction; cytosine metabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; cytidine deamination; pyrimidine nucleoside salvage; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage