LDHA, 1-332aa, Human, His tag, E Coli | MyBioSource MBS203659 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

LDHA, 1-332aa, Human, His tag, E Coli

catalog :

MBS203659

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203659

products type :

Recombinant Protein

products full name :

LDHA, 1-332aa, Human, His tag, E Coli

products short name :

LDHA

products name syn :

GSD11; LDH1; LDHM; PIG19; L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein; LDH muscle subunit; M LDH; L lactate dehydrogenase A chain; l7R2; Lactate dehydrogenase 1; A chain; Lactate dehydrogenase A; Lactate dehydrogenase A4; Lactate dehydrogenase M; LDH muscle subunit; OTTMuSP00000017774; Proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59.

other names :

L-lactate dehydrogenase A chain isoform 1; L-lactate dehydrogenase A chain; L-lactate dehydrogenase A chain; LDH muscle subunit; LDH-A; LDH-M; cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; lactate dehydrogenase M; renal carcinoma antigen NY-REN-59; lactate dehydrogenase A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59

products gene name :

LDHA

other gene names :

LDHA; LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P; LDH-A; LDH-M

uniprot entry name :

LDHA_HUMAN

sequence length :

332

sequence :

MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF

purity :

> 95% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is > 20 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C. Assay: 1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin. 2. Equilibrate to 37C and monitor at A340nm until the value is constant using a spectrophotometer . 3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

products categories :

Redox Proteins

products description :

LDHA, also known as L-lactate dehydrogenase A chain, is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. Recombinant LDHA protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Gosti F, et al. (1987) Proc. Natl. Acad. Sci. u.S.A. 84(4):1000-4. Chung FZ, et al. (1985) Biochem. J. 231(3):537-41.

ncbi gi num :

5031857

ncbi acc num :

NP_005557

ncbi gb acc num :

NM_005566.3

ncbi mol weight :

38.8 kDa (352aa), confirmed by MALDI-TOF.

ncbi pathways :

Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231!!Cysteine And Methionine Metabolism Pathway 104488!!Cysteine And Methionine Metabolism Pathway 103421!!Glycolysis / Gluconeogenesis Pathway 82926!!Glycolysis / Gluconeogenesis Pathway 287!!Glycolysis And Gluconeogenesis Pathway 198814!!HIF-1 Signaling Pathway 695200!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Metabolic Pathways 132956

ncbi summary :

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

size :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage