protein

PDE6D, 1-150aa, Human, His tag, E Coli

MBS203656

0.1 mg

375 USD

Recombinant Protein

PDE6D, 1-150aa, Human, His tag, E Coli

PDE6D

PDED; Retinal rod rhodopsin-sensitive cGMP 3'; 5'-cyclic phosphodiesterase subunit delta; phosphodiesterase 6D; cGMP-specific; rod; delta

retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1; Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta; retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta; GMP-PDE delta; protein p17; phosphodiesterase 6D, cGMP-specific, rod, delta; Protein p17

PDE6D

PDE6D; PDE6D; PDED; JBTS22; PDED; GMP-PDE delta

PDE6D_HUMAN

150

MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alphabeta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDEalphabeta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Zhang H., et al, (2007) Proc Natl Acad Sci u S A. 104(21):8857-62. Dekomien., G. et al. (2003) Genet Sel Evol. 35(4):445-56.

4505671

NP_002592

NM_002601.3

18.4 kDa (158aa), confirmed by MALDI-TOF

ARL13B-mediated Ciliary Trafficking Of INPP5E Pathway (1127508); Assembly Of The Primary Cilium Pathway (1127502); Cargo Trafficking To The Periciliary Membrane Pathway (1127504); Organelle Biogenesis And Maintenance Pathway (1127494); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307)

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family. Chromosomal Location of Human Ortholog: 2q35-q36. Cellular Component: cytoplasmic vesicle membrane; cytoskeleton; cytoplasmic vesicle; cytosol. Molecular Function: protein binding; 3 ,5 -cyclic-nucleotide phosphodiesterase activity; GTPase inhibitor activity; Rab GTPase binding. Biological Process: visual perception; metabolic process; organelle organization and biogenesis; response to stimulus; negative regulation of catalytic activity. Disease: Joubert Syndrome 22

0.1 mg

375 USD

0.5 mg

925