SIRT3, 118-399aa, Human, His tag, E Coli | MyBioSource MBS203655 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

SIRT3, 118-399aa, Human, His tag, E Coli

catalog :

MBS203655

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203655

products type :

Recombinant Protein

products full name :

SIRT3, 118-399aa, Human, His tag, E Coli

products short name :

SIRT3

products name syn :

SIR2L3; Sirtuin3; NAD-dependent deacetylase sirtuin-3; mitochondrial

other names :

NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform a; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin type 3; sirtuin 3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3

products gene name :

SIRT3

other gene names :

SIRT3; SIRT3; SIR2L3; SIR2L3; hSIRT3

uniprot entry name :

SIR3_HUMAN

sequence length :

399

sequence :

MGSSHHHHHH SSGLVPRGSH MSDKGKLSLQ DVAELIRARA CQRVVVMVGA GISTPSGIPD FRSPGSGLYS NLQQYDLPYP EAIFELPFFF HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FASLTEAVRS SVPRLLINRD LVGPLAWHPR SRDVAQLGDV VHGVESLVEL LGWTEEMRDL VQRETGKLDG PDK

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

concentration :

1mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cancer

products description :

NAD-dependent deacetylase sirtuin-3, mitochondrial, also known as SIRT3, belongs to the sirtuin family of proteins. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. SIRT3 exhibits NAD+-dependent deacetylase activity in the mitochondria. Over-expression of SIRT3 results in increased levels of the mitochondrial uncoupling protein 1. SIRT3 protein levels are also elevated in certain breast cancers. Recombinant human SIRT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Schewr B., et al. (2002) J Cell Biol. 158(4):647-57. Kim H S., et al. (2010) Cancer Cell. 17(1):41-52.

ncbi gi num :

6912660

ncbi acc num :

NP_036371

ncbi gb acc num :

NM_012239.5

ncbi mol weight :

33.5kDa (303aa) confirmed by MALDI-TOF

ncbi pathways :

Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Energy Metabolism Pathway (198907); Mitochondrial Biogenesis Pathway (1127495); Organelle Biogenesis And Maintenance Pathway (1127494); Signaling Events Mediated By HDAC Class I Pathway (137997); Signaling Events Mediated By HDAC Class III Pathway (137972); Transcriptional Activation Of Mitochondrial Biogenesis Pathway (1127497)

ncbi summary :

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product. Protein type: Deacetylase; Mitochondrial; EC 3.5.1.-. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: membrane; mitochondrion; mitochondrial matrix. Molecular Function: protein binding; enzyme binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD+ ADP-ribosyltransferase activity. Biological Process: mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation; organelle organization and biogenesis; aerobic respiration; aging

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage