uGDH, 1-494aa, Human, His tag, E Coli | MyBioSource MBS203653 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

uGDH, 1-494aa, Human, His tag, E Coli

catalog :

MBS203653

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203653

products type :

Recombinant Protein

products full name :

uGDH, 1-494aa, Human, His tag, E Coli

products short name :

uGDH

products name syn :

GDH; uDP-GlcDH; uDPGDH; uGD; uDP-glucose dehydrogenase; uDP-glucose 6-dehydrogenase

other names :

UDP-glucose 6-dehydrogenase isoform 1; UDP-glucose 6-dehydrogenase; UDP-glucose 6-dehydrogenase; UDP-Glc dehydrogenase; UDP-glucose dehydrogenase; uridine diphospho-glucose dehydrogenase; UDP-glucose 6-dehydrogenase

products gene name :

uGDH

other gene names :

UGDH; UGDH; GDH; UGD; UDPGDH; UDP-GlcDH; UDP-Glc dehydrogenase; UDP-GlcDH; UDPGDH

uniprot entry name :

UGDH_HUMAN

sequence length :

494

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV

purity :

> 95 % by SDS-PAGE

form :

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity: > 0.1 unit/ml. One unit will oxidize 1.0 umol of uDP-glucose to uDP-glucuronic acid per minute at pH 8.0 at 25C. Assay: 1. Prepare a 1470 ul assay buffer. The final concentrations are 93mM Tris-Hcl (pH 8.0), 0.2mM uDPG, and 1mM beta-NAD. 2. Add 20 ul of recombinant protein uGDH with 1 ug, 2 ug and 5 ug in assay buffer. 3. Mix by inversion and load 200 ul of reaction mix in to a plate well. 4. Record the decrease in A340nm for 5 minutes at 25C

products categories :

Enzymes & Proteases

products description :

uGDH is a member of the uDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts uDP-glucose to uDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human uGDH protein, fused to his-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

products references :

Bontemps Y., et al. (2003) J Biol Chem. 278(24):21566-75. Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70(2):401-10.

ncbi gi num :

4507813

ncbi acc num :

NP_003350

ncbi gb acc num :

NM_003359.3

ncbi mol weight :

59.5 kDa (533aa) confirmed by MALDI-TOF

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Ascorbate And Aldarate Metabolism Pathway (82932); Ascorbate And Aldarate Metabolism Pathway (293); Biological Oxidations Pathway (105698); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663)

ncbi summary :

The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

UGDH: Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Carbohydrate Metabolism - pentose and glucuronate interconversions; Oxidoreductase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 1.1.1.22; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - ascorbate and aldarate; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4p15.1. Cellular Component: nucleoplasm; cytosol. Molecular Function: electron carrier activity; UDP-glucose 6-dehydrogenase activity; NAD binding. Biological Process: glycosaminoglycan biosynthetic process; xenobiotic metabolic process; gastrulation with mouth forming second; UDP-glucuronate biosynthetic process; UDP-glucose metabolic process

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage