PRDX4, 38-271aa, Human, His tag, E Coli | MyBioSource MBS203650 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

PRDX4, 38-271aa, Human, His tag, E Coli

catalog :

MBS203650

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203650

products type :

Recombinant Protein

products full name :

PRDX4, 38-271aa, Human, His tag, E Coli

products short name :

PRDX4

products name syn :

PRX-4; AOE37-2; PRDX4; peroxiredoxin 4 Antioxidant enzyme 372; Antioxidant enzyme AOE372; AOE37 2; AOE372; EC 1.11.1.15; Peroxiredoxin4; PRDX 4; PRX 4; Prx IV; PRX4; PrxIV; Thioredoxin dependent peroxide reductase A0372; Thioredoxin peroxidase; Thioredoxin Peroxidase (Antioxidant Enzyme); Thioredoxin peroxidase AO372; TRANK.

other names :

peroxiredoxin-4; Peroxiredoxin-4; peroxiredoxin-4; antioxidant enzyme AOE372; epididymis secretory sperm binding protein Li 97n; peroxiredoxin IV; prx-IV; thioredoxin peroxidase (antioxidant enzyme); thioredoxin peroxidase AO372; thioredoxin-dependent peroxide reductase A0372; peroxiredoxin 4; Antioxidant enzyme AOE372; AOE37-2; Peroxiredoxin IV; Prx-IV; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372

products gene name :

PRDX4

other gene names :

PRDX4; PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n; AOE37-2; Prx-IV

uniprot entry name :

PRDX4_HUMAN

sequence length :

271

sequence :

MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity: approximately 230-310 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute. Assay: 1. Prepare a 50ul reaction mix into a suitable container : . The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2. Add 5 ul of recombinant PRDX4 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3. Incubate at 25C for 2 minutes. 4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) . 7. Record the increase in A475nm.

products categories :

Redox Proteins

products description :

PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

products references :

Jin D.-Y., et al. (1997) J. Biol. Chem. 272:30952-30961. Wang HQ., et al. (2009) FEBS Lett. 583(9):1511-5.

ncbi gi num :

5453549

ncbi acc num :

NP_006397

ncbi gb acc num :

NM_006406.1

ncbi mol weight :

28.8kDa (255aa), confirmed by MALDI-TOF.

ncbi pathways :

Selenium Pathway (198825)

ncbi summary :

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]

uniprot summary :

PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family. Protein type: Mitochondrial; EC 1.11.1.15; Secreted; Oxidoreductase; Secreted, signal peptide. Chromosomal Location of Human Ortholog: Xp22.11. Cellular Component: extracellular space; smooth endoplasmic reticulum; mitochondrion; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; thioredoxin peroxidase activity. Biological Process: extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; cell redox homeostasis; male gonad development; spermatogenesis; I-kappaB phosphorylation

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage