PKM2, 1-531aa, Human, His tag, E Coli | MyBioSource MBS203644 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

PKM2, 1-531aa, Human, His tag, E Coli

catalog :

MBS203644

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203644

products type :

Recombinant Protein

products full name :

PKM2, 1-531aa, Human, His tag, E Coli

products short name :

PKM2

products name syn :

CTHBP; THBP1; p58; M2-PK; Tumor M2-PK; PK2; PK3; PKM; pyruvate kinase; muscle isoform M2 OIP3; PKM; TCB; OPA interacting protein 3; PKM2; Cytosolic thyroid hormone binding protein; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Thyroid hormone binding protein cytosolic; Tumour Type M2 Pyruvate Kinase; Tumor Type M2 Pyruvate Kinase

other names :

pyruvate kinase PKM isoform a; Pyruvate kinase PKM; pyruvate kinase PKM; OIP-3; OPA-interacting protein 3; PK, muscle type; cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; p58; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumor M2-PK; pyruvate kinase, muscle; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58

products gene name :

PKM2

other gene names :

PKM; PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1

uniprot entry name :

KPYM_HUMAN

sequence length :

531

sequence :

MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity: > 5.0 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37 degre C. Assay: 1. Prepare a 150ul reaction mixture into a suitable container. . - Reaction mixture: 72mM Imidazole (pH 7.5), 7.6mM ADP, 15mM MgCl2, 72mM KCl, 0.2mM Beta-NADH. 2. Incubate for 10 min at 37C. 3. Add 50ul of 20 units/ml LDH, 50ul of 5mM PEP, and incubate for 10 min at 37C. 4. Add 50ul of recombinant PKM2 protein (in 0.1% BSA / PBS) with various concentrations (0.03ug -1ug). 5. Read the decrease in A340nm in kinetic mode for 10 minutes. * PEP (Sigma, Cat# P3637), * LDH

products categories :

Cancer

products description :

PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant human PKM2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Zwerschke W., et al. (2009). Exp Cell Res. 315(16):2765-74. Mazurek S., et al. (2007). Ernst Schering Found Symp Proc. (4):99-124.

ncbi gi num :

33286418

ncbi acc num :

NP_002645

ncbi gb acc num :

NM_002654.4

ncbi mol weight :

60.1 kDa (551aa)

ncbi pathways :

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Disease Pathway (530764); Glucose Metabolism Pathway (106199)

ncbi summary :

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

uniprot summary :

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported. Protein type: Nucleotide Metabolism - purine; EC 2.7.1.40; Mitochondrial; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium. Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding. Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage