protein

Human, His-tagged, Recombinant, E.coli

MBS203642

0.1 mg

285 USD

Recombinant Protein

Human, His-tagged, Recombinant, E.coli

KRAS

C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; NS3; RASK2; GTPase Kras; K-RAS2A; K RAS2B; K RAS4A; K RAS4B; KI RAS; KRAS 1; NS 3

V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; GTPase KRas; GTPase KRas; K-Ras 2; K-ras p21 protein; PR310 c-K-ras oncogene; c-Ki-ras; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; oncogene KRAS2; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; Kirsten rat sarcoma viral oncogene homolog; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-rasGTPase KRas, N-terminally processed

KRAS

KRAS; KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS2; RASK2

RASK_HUMAN

188

MGSSHHHHHH SSGLVPRGSH MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG HEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKC

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl,1mM DTT, 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Cancer

KRAS is a member of the small GTPase superfamily. This protein is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. under normal conditions, Ras family members influence cell growth and differentiation events in a subcellular membrane compartmentalization-based signaling system. Oncogenic Ras can deregulate processes that control both cell proliferation and apoptosis. Recombinant human KRAS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Lee W., et al. (2010) Nature. 465(7297):473-7. Singh M., et al. (2010) Nat Biotechnol. 28(6):585-93.

15488883

AAH13572

23.2 kDa (205aa) confirmed by MALDI-TOF

ARMS-mediated Activation Pathway (106466); Activation Of RAS In B Cells Pathway (576252); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Axon Guidance Pathway (83065)

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: G protein; G protein, monomeric, Ras; Oncoprotein; Motility/polarity/chemotaxis; G protein, monomeric. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft. Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding. Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; response to mineralocorticoid stimulus; social behavior; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; actin cytoskeleton organization and biogenesis; leukocyte migration. Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2

0.1 mg

285 USD

0.5 mg

640