protein

AKR1C1, 1-323aa, Human, His tag, E Coli

MBS203641

0.1 mg

285 USD

Recombinant Protein

AKR1C1, 1-323aa, Human, His tag, E Coli

AKR1C1

20-alpha-HSD; DD1/DD2; HBAB; DDH; DDH1; Aldo-keto reductase family 1; member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase; 20 alpha hydroxysteroid dehydrogenase; 2ALPHAHSD; AK1C1; MBAB; Aldo keto reductase family 1 member C1; C9; Chlordecone reductase homolog; Chlordecone reductase homolog HAKRC; DD1; Dihydrodiol dehydrogenase 1; Dihydrodiol dehydrogenase 1/2; H37; HAKRC; Hepatic dihydrodiol dehydrogenase; High affinity hepatic bile acid-binding protein; Trans-1; 2 dihydrobenzene 1; 2 diol dehydrogenase; Type II 3 alpha hydroxysteroid dehydrogenase

aldo-keto reductase family 1 member C1; Aldo-keto reductase family 1 member C1; aldo-keto reductase family 1 member C1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-hydroxysteroid dehydrogenase; aldo-keto reductase C; chlordecone reductase homolog HAKRC; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; dihydrodiol dehydrogenase isoform DD1; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase; aldo-keto reductase family 1, member C1; 20-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.149); 20-alpha-HSD; Chlordecone reductase homolog HAKRC; Dihydrodiol dehydrogenase 1/2; DD1/DD2; High-affinity hepatic bile acid-binding protein; HBAB; Indanol dehydrogenase (EC:1.1.1.112); Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20)

AKR1C1

AKR1C1; AKR1C1; C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD; DDH; DDH1; 20-alpha-HSD; DD1/DD2; HBAB

AK1C1_HUMAN

323

MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: approximately 0.15 - 0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein. Assay: 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1C1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.

Redox Proteins

AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Zhang Y., et al. (2000) J. Mol. Endocrinol. 25:221-228. Zhang Y., et al. (2009) Mol Cell Endicrinol. 298(1-2):76-83.

5453543

NP_001344

NM_001353.5

38.9 kDa (343aa), confirmed by MALDI-TOF.

Benzo(a)pyrene Metabolism Pathway 198911!!Bile Acid And Bile Salt Metabolism Pathway 106144!!Disease Pathway 530764!!Diseases Associated With Visual Transduction Pathway 771581!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Metabolism Of Xenobiotics By Cytochrome P450 Pathway 83031!!Metabolism Of Xenobiotics By Cytochrome P450 Pathway 425!!Retinoid Metabolism And Transport Pathway 187208!!Signal Transduction Pathway 477114

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

0.1 mg

285 USD