catalog number :
MBS203619
products type :
Recombinant Protein
products full name :
HIV1-p24, 155-321aa, HIV-1, His tag, E Coli
products short name :
HIV1-p24
products name syn :
CA; Capsid protein p24; Group-specific antigen - p24
other names :
gag polyprotein; Gag polyprotein; Pr55GagCleaved into the following 6 chains:Matrix protein p17; MA; Capsid protein p24; CA; Spacer peptide p2; Nucleocapsid protein p7; NC; Spacer peptide p1; p6-gag
products gene name :
HIV1-p24
other gene names :
gag; MA; CA; NC
uniprot entry name :
GAG_HV1N5
sequence :
MGSSHHHHHH SSGLVPRGSH MWVKVVEEKA FSPEVIPMFS ALSEGATPQD LNTMLNTVGG HQAAMQMLKE TINEEAAEWD RLHPVHAGPI APGQMREPRG SDIAGTTSTL QEQIGWMTHN PPIPVGEIYK RWIILGLNKI VRMYSPTSIL DIRQGPKEPF RDYVDRFYKT LRAEQASQEV KNWMTETL
purity :
> 80% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 100 mM NaCl, 0.1 mM PMSF, 10% Glycerol
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: HIV-1. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Virus
products description :
The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Bernard NF., et al. (2009) Clin Immunol. 131(2):277-87. Wagstaff M., et al. (1995) Immunology. 86(1):85-96.
ncbi mol weight :
21.2kDa(188aa), confirmed by MALDI-TOF