Thymidylate synthase, 1-313aa, Human, His tag, E Coli | MyBioSource MBS203615 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Thymidylate synthase, 1-313aa, Human, His tag, E Coli

catalog :

MBS203615

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203615

products type :

Recombinant Protein

products full name :

Thymidylate synthase, 1-313aa, Human, His tag, E Coli

products short name :

Thymidylate synthase

products name syn :

HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthase; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase

other names :

thymidylate synthase; Thymidylate synthase; thymidylate synthase; TSase; thymidylate synthetase

other gene names :

TYMS; TYMS; TS; TMS; HST422; TS; TS; TSase

uniprot entry name :

TYSY_HUMAN

sequence length :

313

sequence :

MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP(thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu). Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Chiu TH., et al. (2009) Anticancer Res. 29(11):4503-11. Pena MM., et al. (2009) J Biol Chem. 284(46):31597-607.

ncbi gi num :

4507751

ncbi acc num :

NP_001062

ncbi gb acc num :

NM_001071.2

ncbi mol weight :

37.8 kDa (333aa), confirmed by MALDI-TOF.

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); E2F Mediated Regulation Of DNA Replication Pathway (105778); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); G1/S Transition Pathway (105769); G1/S-Specific Transcription Pathway (105776); Integrated Pancreatic Cancer Pathway (711360); Metabolic Pathways (132956); Metabolism Pathway (477135)

ncbi summary :

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

uniprot summary :

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer. Protein type: DNA repair, damage; EC 2.1.1.45; Methyltransferase; Mitochondrial; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate. Chromosomal Location of Human Ortholog: 18p11.32. Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; nucleolus; nucleus; cytosol. Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding. Biological Process: immortalization of host cell by virus; circadian rhythm; methylation; uracil metabolic process; tetrahydrofolate metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; response to cytokine stimulus; cartilage development; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage