protein

SQSTM1, 1-356aa, Human, His-tag, Recombinant, E Coli

MBS203612

0.1 mg

285 USD

Recombinant Protein

SQSTM1, 1-356aa, Human, His-tag, Recombinant, E Coli

SQSTM1

A170; OSIL; p60; p62; p62B; PDB3; ZIP3; Sequestosome 1 EBI 3 associated protein of 60 kDa; ZIP; ZIP 3; EBI3 associated protein of 60 kDa; EBIAP; MGC127197; ORCA.M530; OSF-6; Osi; Oxidative stress induced like; Paget disease of bone 3; PDB 3; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; Phosphotyrosine independent ligand for the Lck SH2 domain p62; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; SQSTM 1; STAP; STONE14; ubiquitin binding protein p62

sequestosome-1 isoform 2; Sequestosome-1; sequestosome-1; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; ubiquitin-binding protein p62; sequestosome 1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62

SQSTM1

SQSTM1; SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; ORCA; OSIL; EBIAP; p60

SQSTM_HUMAN

356

MAMSYVKDDI FRIYIKEKKE CRRDHRPPCA QEAPRNMVHP NVICDGCNGP VVGTRYKCSV CPDYDLCSVC EGKGLHRGHT KLAFPSPFGH LSEGFSHSRW LRKVKHGHFG WPGWEMGPPG NWSPRPPRAG EARPGPTAES ASGPSEDPSV NFLKNVGESV AAALSPLGIE VDIDVEHGGK RSRLTPVSPE SSSTEEKSSS QPSSCCSDPS KPGGNVEGAT QSLAEQMRKI ALESEGRPEE QMESDNCSGG DDDWTHLSSK EVDPSTGELQ SLQMPESEGP SSLDPSQEGP TGLKEAALYP HLPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KHPPPLLEHH HHHH

> 90% by SDS-PAGE

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (uBA) domain of the sequestosome 1 protein commonly cause Paget's disease since the uBA is necessary for aggregate sequestration and cell survival. Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Falchetti A., et al. (2004) J Bone Miner Res. 19(6):1013-7.

214830451

NP_001135771

NM_001142299.1

39.7 kDa (364aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

BDNF Signaling Pathway (712093); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Cytokine Signaling In Immune System Pathway (366171); IL1-mediated Signaling Events Pathway (137944); Immune System Pathway (106386); Interleukin-1 Signaling Pathway (160141); NF-kB Is Activated And Signals Survival Pathway (106450); NRIF Signals Cell Death From The Nucleus Pathway (106445); Neurotrophic Factor-mediated Trk Receptor Signaling Pathway (137977); Osteoclast Differentiation Pathway (193147)

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer s disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Autophagy; Ubiquitin conjugating system; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 5q35. Cellular Component: nucleoplasm; PML body; endoplasmic reticulum; lysosome; late endosome; cytoplasm; autophagic vacuole; cytoplasmic vesicle; inclusion body; cytosol. Molecular Function: identical protein binding; protein serine/threonine kinase activity; ubiquitin binding; protein binding; protein homodimerization activity; protein kinase C binding; zinc ion binding; SH2 domain binding; receptor tyrosine kinase binding; protein kinase binding. Biological Process: ubiquitin-dependent protein catabolic process; regulation of Ras protein signal transduction; nerve growth factor receptor signaling pathway; macroautophagy; immune system process; positive regulation of apoptosis; protein heterooligomerization; endosome transport; protein amino acid phosphorylation; protein localization; response to stress; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macroautophagy; negative regulation of apoptosis. Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone

0.1 mg

285 USD

0.5 mg

640