protein

NME2, 1-152aa, Human, E Coli

MBS203610

0.1 mg

285 USD

Recombinant Protein

NME2, 1-152aa, Human, E Coli

NME2

NDPK-B; NDPKB; NM23-H2; NM23B; Nucleoside diphosphate kinase B C myc purine binding transcription factor PuF; NDK B; NDP kinase B; NM23 H2; Non-Metastatic Cells 2; MGC111212

nucleoside diphosphate kinase B isoform a; Nucleoside diphosphate kinase B; nucleoside diphosphate kinase B; HEL-S-155an; NDP kinase B; c-myc purine-binding transcription factor PUF; c-myc transcription factor; epididymis secretory sperm binding protein Li 155an; histidine protein kinase NDKB; non-metastatic cells 2, protein (NM23) expressed in; non-metastatic cells 2, protein (NM23B) expressed in; nucleotide diphosphate kinase B; NME/NM23 nucleoside diphosphate kinase 2; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB (EC:2.7.13.3); nm23-H2

NME2

NME2; NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2; NM23B; NDK B; NDP kinase B

NDKB_HUMAN

152

MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli

Metabolism

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Munkonge FM., et al. (2009) J Biol Chem. 284(39):26978-87. Treharne KJ., et al. (2009) FEBS Lett. 583(17):2789-92.

66392227

NP_001018149

NM_001018139.2

17.2 kDa (152aa), confirmed by MALDI-TOF.

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); CMP Phosphorylation Pathway (782393); CMP Phosphorylation Pathway (908070); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (618581); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (468243); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nucleotide Metabolism Pathway (198876)

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; Kinase, other; EC 2.7.13.3; Nuclear receptor co-regulator; EC 2.7.4.6; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Other group; NDK family. Chromosomal Location of Human Ortholog: 17q21.3. Cellular Component: ruffle; centrosome; focal adhesion; mitochondrion; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; cytosol; nucleus. Molecular Function: gamma-tubulin binding; protein binding; protein histidine kinase activity; metal ion binding; nucleoside diphosphate kinase activity; intermediate filament binding; protein kinase binding; transcription factor activity; single-stranded DNA binding; ATP binding. Biological Process: GTP biosynthetic process; integrin-mediated signaling pathway; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; negative regulation of myeloid leukocyte differentiation; UTP biosynthetic process; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; regulation of epidermis development; positive regulation of epithelial cell proliferation; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; negative regulation of apoptosis; response to amine stimulus

0.1 mg

285 USD

0.5 mg

640