protein

HRAS, 1-186aa, Human, His-tag, E Coli

MBS203609

0.1 mg

285 USD

Recombinant Protein

HRAS, 1-186aa, Human, His-tag, E Coli

HRAS

HMG14; GC104230; v-Ha-ras Harvey rat sarcoma viral oncogene homolog C BAS/HAS; c H ras; C HA RAS1; c has/bas p21 protein; c ras Ki 2 activated oncogene; CTLO; GTP and GDP binding peptide B; H Ras 1; H RASIDX; Ha Ras; Ha Ras1 proto oncoprotein; HAMSV; HRAS; HRAS1; K ras; N ras; p19 H RasIDX protein; p21ras; Ras family small GTP binding protein H Ras; RASH1; Transformation gene oncogene HAMSV; Transforming protein p21; v Ha ras Harvey rat sarcoma viral oncogene homolog; VH Ras; vHa RAS.

GTPase HRas isoform 1; GTPase HRas; GTPase HRas; GTP- and GDP-binding peptide B; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncoprotein; Ras family small GTP binding protein H-Ras; c-has/bas p21 protein; c-ras-Ki-2 activated oncogene; p19 H-RasIDX protein; transformation gene: oncogene HAMSV; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncogene homolog; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21rasGTPase HRas, N-terminally processed

HRAS

HRAS; HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; HRAS1

RASH_HUMAN

189

MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCLEHH HHHH

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

HRAS, also known as GTPase HRas, belongs to the Ras family which function in signal transduction pathways. This protein can bind GTP and GDP, and it has intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Recombinant human HRAS protein was expressed in E.coli and purified by using conventional chromatography techniques.

McCormick F. (1995) Mol Reprod Dev. 42(4):500-6. Ayllon V. et al. (2000) Mol Membr Biol. 17(2):65-73.

194363762

NP_001123914

NM_001130442.1

22.0 kDa (194aa), confirmed by MALDI-TOF.

ARMS-mediated Activation Pathway (106466); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Activation Of RAS In B Cells Pathway (576252); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]

HRas: a small GTPase protein of the Ras family. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Mutations are implicated in a variety of human tumors. Protein type: Oncoprotein; G protein, monomeric, Ras; Motility/polarity/chemotaxis; G protein, monomeric; G protein. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus. Molecular Function: protein C-terminus binding; protein binding; GTP binding. Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; positive regulation of JNK cascade; chemotaxis; signal transduction; negative regulation of cell proliferation; synaptic transmission; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; small GTPase mediated signal transduction; positive regulation of cell proliferation; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; cell cycle arrest; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; mitotic cell cycle checkpoint; MAPKKK cascade; endocytosis; social behavior; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; cell proliferation; negative regulation of cell differentiation; organ morphogenesis; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; blood coagulation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of cell migration. Disease: Bladder Cancer; Thyroid Carcinoma, Follicular; Costello Syndrome; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Nevus, Epidermal

0.1 mg

285 USD

0.5 mg

640