Alcohol dehydrogenase, 1-325aa, Human, E Coli | MyBioSource MBS203604 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Alcohol dehydrogenase, 1-325aa, Human, E Coli

catalog :

MBS203604

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203604

products type :

Recombinant Protein

products full name :

Alcohol dehydrogenase, 1-325aa, Human, E Coli

products short name :

Alcohol dehydrogenase

products name syn :

AKR1A1; ALDR1; ALR; ARM; DD3; Alcohol dehydrogenase [NADP+] Alcohol dehydrogenase; Aldehyde reductase; aldo-keto reductase family 1 member A1 (aldehyde reductase).

other names :

alcohol dehydrogenase; Alcohol dehydrogenase [NADP(+)]; alcohol dehydrogenase [NADP(+)]; alcohol dehydrogenase [NADP(+)]; HEL-S-165mP; aldehyde reductase; aldo-keto reductase family 1 member A1; dihydrodiol dehydrogenase 3; epididymis secretory protein Li 6; epididymis secretory sperm binding protein Li 165mP; aldo-keto reductase family 1, member A1 (aldehyde reductase); Aldehyde reductase; Aldo-keto reductase family 1 member A1

other gene names :

AKR1A1; AKR1A1; ALR; ARM; DD3; ALDR1; HEL-S-6; ALDR1; ALR

uniprot entry name :

AK1A1_HUMAN

sequence length :

325

sequence :

MAASCVLLHT GQKMPLIGLG TWKSEPGQVK AAVKYALSVG YRHIDCAAIY GNEPEIGEAL KEDVGPGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTICYDSTHY KETWKALEAL VAKGLVQALG LSNFNSRQID DILSVASVRP AVLQVECHPY LAQNELIAHC QARGLEVTAY SPLGSSDRAW RDPDEPVLLE EPVVLALAEKYGRSPAQILL RWQVQRKVIC IPKSITPSRI LQNIKVFDFT FSPEEMKQLN ALNKNWRYIV PMLTVDGKRV PRDAGHPLYP FNDPY

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli

products categories :

Redox Proteins

products description :

Alcohol dehydrogenase [NADP+] (AKR1A1) is a member of the aldo/keto reductase superfamily and catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. This protein is closely related (65% identity) to aldose reductase, an enzyme involved in the pathogenesis of some diabetic and galactosemic complications. It plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAuN). Recombinant human Alcohol dehydrogenase was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Bohren KM., et al. (1989) J Biol Chem. 264(16): 9547-51. Vander Jaqt DL., et al. (1990) J Biol Chem. 265(19):10912-8.

ncbi gi num :

5174391

ncbi acc num :

NP_006057

ncbi gb acc num :

NM_006066.3

ncbi mol weight :

36.5 kDa (325aa), confirmed by MALDI-TOF.

ncbi pathways :

Benzo(a)pyrene Metabolism Pathway (198911); Degradation Of Aromatic Compounds Pathway (843021); Degradation Of Aromatic Compounds Pathway (717401); Glucuronate Pathway (uronate Pathway) (997799); Glucuronate Pathway (uronate Pathway) (468207); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Metabolic Pathways (132956)

ncbi summary :

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]

uniprot summary :

AKR1A1: Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4- nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D- glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Belongs to the aldo/keto reductase family. Protein type: Oxidoreductase; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.2. Chromosomal Location of Human Ortholog: 1p33-p32. Cellular Component: extracellular space; apical plasma membrane; cytosol. Molecular Function: L-glucuronate reductase activity; electron carrier activity; aldehyde reductase activity. Biological Process: aldehyde catabolic process; L-ascorbic acid biosynthetic process; D-glucuronate catabolic process; glucose metabolic process; aldehyde metabolic process

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage