protein

PDIA3, 25-505aa, Human, His tag, E Coli

MBS203583

0.1 mg

285 USD

Recombinant Protein

PDIA3, 25-505aa, Human, His tag, E Coli

PDIA3

ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; Protein disulfide-isomerase A3 58 kDa glucose regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; ER p57; ERp 57; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; HsT17083; PDIA 3; PDIA3; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3.

protein disulfide-isomerase A3; Protein disulfide-isomerase A3; protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER protein 57; ER protein 60; disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; glucose regulated protein, 58kDa; phospholipase C-alpha; protein disulfide isomerase-associated 3; protein disulfide isomerase family A, member 3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60

PDIA3

PDIA3; PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n; ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60

PDIA3_HUMAN

505

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Heat Shock Proteins

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Vigneron N., et al. (2009) Eur J Immunol. 39(9):2371-6. Forster ML., et al. (2009) J Biol Chem. 284(19):13045-56.

21361657

NP_005304

NM_005313.4

58.5 kDa (518aa), confirmed by MALDI-TOF.

Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Asparagine N-linked Glycosylation Pathway (161013); Calnexin/calreticulin Cycle Pathway (187218); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway (1127605); Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway (1127608)

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway. Protein type: Endoplasmic reticulum; Apoptosis; Phospholipase; Isomerase; Chaperone; EC 5.3.4.1; Protease. Chromosomal Location of Human Ortholog: 15q15. Cellular Component: smooth endoplasmic reticulum; focal adhesion; cell surface; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus. Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity. Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; cell redox homeostasis; protein import into nucleus; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; proteolysis; signal transduction; protein retention in ER

0.1 mg

285 USD

0.5 mg

640