This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
protein
product name :
NFNB, 1-217aa, E Coli, His tag, E Coli
catalog :
MBS203581
quantity :
0.1 mg
price :
310 USD
product information
catalog number :
MBS203581
products type :
Recombinant Protein
products full name :
NFNB, 1-217aa, E Coli, His tag, E Coli
products short name :
NFNB
products name syn :
dprA; nfsB; nfsI; ntr; dihydropteridine reductase; NAD(P)H-dependent; oxygen-insensitive
other names :
dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive; Oxygen-insensitive NAD(P)H nitroreductase; dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive; Dihydropteridine reductase (EC:1.5.1.34); FMN-dependent nitroreductase
products gene name :
NFNB
other gene names :
nfsB; nfnB; dprA; ECK0570; JW0567; nfnB; nfsI; ntr; dprA; nfsB; nfsI; ntr
uniprot entry name :
NFNB_ECOLI
sequence length :
217
sequence :
MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Redox Proteins
products description :
NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Jarrom D., et al. (2009) Biochemistry. 48(32):7665-72. Singleton DC., et al. (2007) Cancer Gene Ther. 14(12):953-67.
ncbi gi num :
16128561
ncbi acc num :
NP_415110
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P38489
ncbi mol weight :
26.0 kDa (237aa) confirmed by MALDI-TOF
ncbi pathways :
Microbial Metabolism In Diverse Environments Pathway 179780!!Nitrotoluene Degradation Pathway 1078!!Nitrotoluene Degradation Pathway 386
ncbi summary :
nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG20151]. Aspartate 37 has been implicated in binding the NADH cofactor. [More information is available at EcoCyc: EG50005].
size :
0.1 mg
price :
310 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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