TSG101, 1-145aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203576 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TSG101, 1-145aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203576

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203576

products type :

Recombinant Protein

products full name :

TSG101, 1-145aa, Human, His-tag, Recombinant, E Coli

products short name :

TSG101

products name syn :

TSG10; VPS23; Tumor susceptibility gene 101 ESCRT I complex subunit TSG101; TSG 10; TSG 101; Tumor susceptibility gene 10; Tumor susceptibility gene 101; Tumor susceptibility protein; Tumor susceptibility protein isoform 3; VPS 23; VPS23.

other names :

tumor susceptibility gene 101 protein; Tumor susceptibility gene 101 protein; tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; tumor susceptibility protein; tumor susceptibility 101; ESCRT-I complex subunit TSG101

products gene name :

TSG101

other gene names :

TSG101; TSG101; TSG10; VPS23

uniprot entry name :

TS101_HUMAN

sequence length :

390

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cell Cycle

products description :

TSG101(Tumor susceptibility gene 101) protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. This protein contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. Mutations and alternative splicing in this protein occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. Recombinant human TSG101 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Vardhana S., et al. (2010) Immunity. 32(4):531-40. Popov S., et al. (2009) J Virol. 83(14):7185-93.

ncbi gi num :

5454140

ncbi acc num :

NP_006283

ncbi gb acc num :

NM_006292.3

ncbi mol weight :

20.7 kDa (181aa), confirmed by MALDI-TOF

ncbi pathways :

Assembly Of The HIV Virion Pathway (106008); Budding And Maturation Of HIV Virion Pathway (106009); Disease Pathway (530764); ESCRT-I Complex Pathway (413473); ESCRT-I Complex Pathway (890624); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Endosomal Sorting Complex Required For Transport (ESCRT) Pathway (205244); Glucocorticoid Receptor Regulatory Network Pathway (138014); HIV Infection Pathway (105983)

ncbi summary :

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

uniprot summary :

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 11p15. Cellular Component: multivesicular body; late endosome membrane; late endosome; early endosome; cytoplasm; nucleolus; plasma membrane; endosome membrane. Molecular Function: protein binding; ubiquitin binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding. Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral reproduction; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; negative regulation of transcription, DNA-dependent; cell cycle arrest. Disease: Breast Cancer

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage