HEXIM1, 1-359aa, Human, His tag, E Coli | MyBioSource MBS203572 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

HEXIM1, 1-359aa, Human, His tag, E Coli

catalog :

MBS203572

quantity :

0.1 mg

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203572

products type :

Recombinant Protein

products full name :

HEXIM1, 1-359aa, Human, His tag, E Coli

products short name :

HEXIM1

products name syn :

CLP1; EDG1; HIS1; MAQ1; Hexamethylene bis-acetamide inducible 1 Cardiac lineage protein 1; CLP 1; EDG 1; FLJ13562; Hexamethylene bis acetamide inducible 1; Hexamethylene bis acetamide inducible protein; Hexamethylene bis acetamide inducible transcript 1; HEXIM 1; HEXIM1 protein; HIS 1; HMBA inducible; MAQ 1; Menage a quatre 1.

other names :

protein HEXIM1; Protein HEXIM1; protein HEXIM1; HMBA-inducible; cardiac lineage protein 1; estrogen down-regulated gene 1 protein; hexamethylene bis-acetamide-inducible protein 1; hexamethylene bisacetamide-inducible protein; hexamethylene-bis-acetamide-inducible transcript 1; hexamthylene bis-acetamide inducible 1; menage a quatre 1; menage a quatre protein 1; hexamethylene bis-acetamide inducible 1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1

products gene name :

HEXIM1

other gene names :

HEXIM1; HEXIM1; CLP1; EDG1; HIS1; MAQ1; CLP1; EDG1; HIS1; MAQ1

uniprot entry name :

HEXI1_HUMAN

sequence length :

359

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Transcription & Translation

products description :

HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation. Recombinant HEXIM1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Shimizu N., et al. (2005) Proc. Natl. Acad. Sci. u.S.A. 102(24):8555-60. Li Q., et al. (2007) Nucleic Acids Res 35(8):2503-12.

ncbi gi num :

5453682

ncbi acc num :

NP_006451

ncbi gb acc num :

NM_006460.2

ncbi mol weight :

44.8 kDa (396aa), confirmed by MALDI-TOF.

ncbi summary :

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]

uniprot summary :

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family. Protein type: Motility/polarity/chemotaxis; Transcription factor. Chromosomal Location of Human Ortholog: 17q21.31. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding. Biological Process: transcription, DNA-dependent; heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

size1 :

0.1 mg

price1 :

510 USD

size2 :

0.5 mg

price2 :

1335

more info or order :

MyBioSource product webpage