protein

SurA, 21-428aa, E Coli, His tag, E Coli

MBS203564

0.1 mg

310 USD

Recombinant Protein

SurA, 21-428aa, E Coli, His tag, E Coli

SurA

PPIase surA; Rotamase surA; Survival protein A; Chaperone surA

peptidyl-prolyl cis-trans isomerase (PPIase); Chaperone SurA; peptidyl-prolyl cis-trans isomerase (PPIase); Peptidyl-prolyl cis-trans isomerase SurA (EC:5.2.1.8); PPIase SurA; Rotamase SurA; Survival protein A

SurA

surA; surA; ECK0054; JW0052; PPIase SurA

SURA_ECOLI

428

MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: E Coli. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 450 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant surA protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

Heat Shock Proteins

SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E.coli in stationary phase and required for pilus biogenesis. Recombinant E.coli SurA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Virlogeux-Payant I., et al (2009) Microbiology. 155(Pt 5):1613-22. Kolter R., et al. (1996) J Bacteriol. 178(6):1770-3.

16128047

NP_414595

NC_000913.3

P0ABZ6

47.3 kDa(429aa), confirmed by MALDI-TOF

Parvulin (ppiC) family. [More information is available at EcoGene: EG10985]. SurA in Escherichia coli is a periplasmic peptidyl-prolyl isomerase which is necessary for the proper folding of outer membrane proteins including OmpA, OmpF and LamB. [More information is available at EcoCyc: EG10985].

0.1 mg

310 USD