protein

CCS, 1-274aa, Human, His tag, E Coli

MBS203559

0.1 mg

310 USD

Recombinant Protein

CCS, 1-274aa, Human, His tag, E Coli

CCS

Copper chaperone for superoxide dismutase

copper chaperone for superoxide dismutase; Copper chaperone for superoxide dismutase; copper chaperone for superoxide dismutase; superoxide dismutase copper chaperone; copper chaperone for superoxide dismutase; Superoxide dismutase copper chaperone

CCS

CCS; CCS

CCS_HUMAN

274

MGSSHHHHHH SSGLVPRGSH MASDSGNQGT LCTLEFAVQM TCQSCVDAVR KSLQGVAGVQ DVEVHLEDQM VLVHTTLPSQ EVQALLEGTG RQAVLKGMGS GQLQNLGAAV AILGGPGTVQ GVVRFLQLTP ERCLIEGTID GLEPGLHGLH VHQYGDLTNN CNSCGNHFNP DGASHGGPQD SDRHRGDLGN VRADADGRAI FRMEDEQLKV WDVIGRSLII DEGEDDLGRG GHPLSKITGN SGERLACGII ARSAGLFQNP KQICSCDGLT IWEERGRPIA GKGRKESAQP PAHL

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Cancer

CCS is essential for the incorporation of copper into SOD-1, and therefore is necessary for its enzymatic activity. CCS prevents copper ions from binding to intracellular copper scavengers and provides the SOD-1 enzyme with the necessary copper cofactor. CCS escorts copper only to SOD-1 and fails to deliver copper to proteins in the mitochondria, nucleus or secretory pathway. While many tissues express CCS, the chaperone is most abundant in the kidney, liver and Purkinje cells in the neuropil of the central nervous system. Recombinant human CCS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Romerius P., et al. (2010) Clin Cancer Res. 16(15):3843-50. Seetharaman SV., et al. (2010) Biochemistry. 49(27):5714-25.

4826665

NP_005116

NM_005125.1

31.2 kDa (294aa), confirmed by MALDI-TOF

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552)

Copper chaperone for superoxide dismutase specifically delivers Cu to copper/zinc superoxide dismutase and may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor. [provided by RefSeq, Jul 2008]

CCS: Delivers copper to copper zinc superoxide dismutase (SOD1). Chromosomal Location of Human Ortholog: 11q13. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: copper ion transmembrane transporter activity; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity; protein disulfide oxidoreductase activity. Biological Process: response to reactive oxygen species; removal of superoxide radicals; superoxide metabolic process; intracellular copper ion transport; positive regulation of oxidoreductase activity

0.1 mg

310 USD

0.5 mg

720