protein

CRYM, 1-314aa, Human, His tag, E Coli

MBS203551

0.1 mg

285 USD

Recombinant Protein

CRYM, 1-314aa, Human, His tag, E Coli

CRYM

THBP; Crystallin mu CRYM; DFNA 40; DFNA40; Mu crystallin homolog; NADP regulated thyroid hormone binding protein; OTTHuMP00000115878.

ketimine reductase mu-crystallin; Ketimine reductase mu-crystallin; ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone binding protein; mu-crystallin homolog; thiomorpholine-carboxylate dehydrogenase; crystallin, mu; NADP-regulated thyroid-hormone-binding protein

CRYM

CRYM; CRYM; THBP; DFNA40; THBP

CRYM_HUMAN

314

MGSSHHHHHH SSGLVPRGSH MSRVPAFLSA AEVEEHLRSS SLLIPPLETA LANFSSGPEG GVMQPVRTVV PVTKHRGYLG VMPAYSAAED ALTTKLVTFY EDRGITSVVP SHQATVLLFE PSNGTLLAVM DGNVITAKRT AAVSAIATKF LKPPSSEVLC ILGAGVQAYS HYEIFTEQFS FKEVRIWNRT KENAEKFADT VQGEVRVCSS VQEAVAGADV IITVTLATEP ILFGEWVKPG AHINAVGASR PDWRELDDEL MKEAVLYVDS QEAALKESGD VLLSGAEIFA ELGEVIKGVK PAHCEKTTVF KSLGMAVEDT VAAKLIYDSW SSGK

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Neuroscience

Crystallin mu, also known as CRYM, is a member of the crystallin protein family. Crystallins are separated into two classes, taxon-specific and ubiquitous. This gene encodes a taxon-specific crystallin protein. The human CRYM gene maps to chromosome 16p13.11, and encodes a protein that is expressed in neural tissue, muscle, and kidney. unlike other crystallins, CRYM does not perform a structural role in lens tissue, but rather it binds NADPH and thyroid hormone, which indicates that it may have other regulatory or developmental functions. Recombinant human CRYM, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Kim RY., et al. (1992). Proc Natl Acad Sci uSA. 89(19):9292-6. Muaders NW., et al. (1988) J Biol Chem. 263:15462-15466.

4503065

NP_001879

NM_001888.4

35.9 kDa (334aa), confirmed by MALDI-TOF

Lysine Degradation II (pipecolate Pathway) (1108775); Lysine Degradation II (pipecolate Pathway) (1108790); Superpathway Of Lysine Degradation (139177)

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]

CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family. Protein type: EC 1.5.1.25; Lyase. Chromosomal Location of Human Ortholog: 16p12.2. Cellular Component: mitochondrion; cytoplasm; nucleus. Molecular Function: thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity. Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process. Disease: Deafness, Autosomal Dominant 40

0.1 mg

285 USD

0.5 mg

640