protein

Serpin A5, 20-406aa, Human, His-tag, E Coli

MBS203548

0.05 mg

375 USD

Recombinant Protein

Serpin A5, 20-406aa, Human, His-tag, E Coli

Serpin A5

PAI3; PCI; PROCI; Protein C inhibitor; Serpin peptidase inhibitor; clade A; member 5 Acrosomal serine protease inhibitor; IPSP; PAI 3; PLANH 3; PLANH3; Plasma serine protease inhibitor; Plasminogen activator inhibitor 3; Plasminogen activator inhibitor III; Serpin A5; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5; Serpin peptidase inhibitor clade A member 5; SerpinA5.

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5; Plasma serine protease inhibitor; plasma serine protease inhibitor; acrosomal serine protease inhibitor; plasminogen activator inhibitor III; plasminogen activator inhibitor-3; protein C inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5; Acrosomal serine protease inhibitor; Plasminogen activator inhibitor 3; PAI-3; PAI3; Protein C inhibitor; PCI; Serpin A5

SERPINA5; SERPINA5; PCI; PAI3; PAI-3; PCI-B; PROCI; PLANH3; PCI; PLANH3; PROCI; PAI-3; PAI3; PCI

IPSP_HUMAN

406

MGSSHHHHHH SSGLVPRGSH MHRHHPREMK KRVEDLHVGA TVAPSSRRDF TFDLYRALAS AAPSQNIFFS PVSISMSLAM LSLGAGSSTK MQILEGLGLN LQKSSEKELH RGFQQLLQEL NQPRDGFQLS LGNALFTDLV VDLQDTFVSA MKTLYLADTF PTNFRDSAGA MKQINDYVAK QTKGKIVDLL KNLDSNAVVI MVNYIFFKAK WETSFNHKGT QEQDFYVTSE TVVRVPMMSR EDQYHYLLDR NLSCRVVGVP YQGNATALFI LPSEGKMQQV ENGLSEKTLR KWLKMFKKRQ LELYLPKFSI EGSYQLEKVL PSLGISNVFT SHADLSGISN HSNIQVSEMV HKAVVEVDES GTRAAAATGT IFTFRSARLN SQRLVFNRPF LMFIVDNNIL FLGKVNRP

> 90% by SDS-PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Proteases & Inhibitors

Serpin A5, also known as PCI (Protein C Inhibitor) or PAI3 (plasminogen activator inhibitor 3), is a member of the serpin serine proteinase inhibitor family. This protein inhibits plasminogen activators as well as activated protein C. Serpin A5 is secreted in plasma, but is also expressed in liver. Serpin A5 is involved in cell inflammation, proliferation, apoptosis, tumour cell migration, invasion, and metastasis. Recombinant Serpin A5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Han MH., et al. (2008) Nature. 451(7182):1076-81. Li W., et al. (2008) J Biol Chem. 283(51):36039-45.

14286238

AAH08915

45.9 kDa (408aa), confirmed by MALDI-TOF.

Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Intrinsic Pathway (106059)

The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. [provided by RefSeq, Aug 2012]

SERPINA5: Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary- type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as an hydrophobic hormone carrier by its binding to retinoic acid. Belongs to the serpin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q32.1. Cellular Component: extracellular space; protein complex; membrane; extracellular region; external side of plasma membrane; acrosomal membrane. Molecular Function: acrosin binding; heparin binding; serine-type endopeptidase inhibitor activity; retinoic acid binding; glycosaminoglycan binding; protease binding; phosphatidylcholine binding. Biological Process: fusion of sperm to egg plasma membrane; spermatogenesis; lipid transport; blood coagulation

0.05 mg

375 USD

0.25 mg

925