Galectin9, 1-148aa, Human, His tag, E. coli | MyBioSource MBS203545 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Galectin9, 1-148aa, Human, His tag, E. coli

catalog :

MBS203545

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203545

products type :

Recombinant Protein

products full name :

Galectin9, 1-148aa, Human, His tag, E. coli

products short name :

Galectin9

products name syn :

LGALS9; Gal-9; Tumor antigen HOM-HD-21; Ecalectin; galectin 9; HOM HD 21; HOMHD21; HuAT; Lectin galactoside binding soluble 9; LGAL S9; LGALS 9; LGALS9A; MGC117375; MGC125973; MGC125974; urate transporter/channel protein; galectin 9

other names :

galectin-9 isoform long; Galectin-9; galectin-9; ecalectin; gal-9; galectin 9; tumor antigen HOM-HD-21; urate transporter/channel protein; lectin, galactoside-binding, soluble, 9; Ecalectin; Tumor antigen HOM-HD-21

other gene names :

LGALS9; LGALS9; HUAT; LGALS9A; Gal-9

uniprot entry name :

LEG9_HUMAN

sequence length :

355

sequence :

MGSSHHHHHH SSGLVPRGSH MAFSGSQAPY LSPAVPFSGT IQGGLQDGLQ ITVNGTVLSS SGTRFAVNFQ TGFSGNDIAF HFNPRFEDGG YVVCNTRQNG SWGPEERKTH MPFQKGMPFD LCFLVQSSDF KVMVNGILFV QYFHRVPFHR VDTISVNGSV QLSYISFQ

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: The ED50 for this effect is is 1.25 - 5 ug/ml. Measured by its ability to agglutinate human red blood cells. Assay: 1. Mix equal volumes of human blood and Alsever's solution (pH 7.0). (Alsever's solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citric acid 0.055 g, D-glucose 2.05g in DW100 ml) . 2. Centrifuge at 15000rpm for 10 minutes and wash four times with PBS. 3. Dilute packed cells in a 0.5 mg/ml trypsin-EDTA solution to give 4% red cell suspension. 4. Incubate for 1h at 37C and wash four times with PBS. 5. Dilute packed cells in PBS to give 4% red cell suspension. 6. Load 50ul of 0.5%BSA-in-0.15M-NaCl solution and 25ul of 4%-Red-Cell-in-PBS in u shaped wells. 7. Add 25ul of serial diluted galectin protein in PBS to each well plate. (Round bottom 96 well plate) . 8. Incubate for 30min at room temperature to observe visible agglutination.

products categories :

Adhesion & Movement

products description :

Galectin-9 (Gal-9) is a member of animal lectins that have an affinity to beta-galactosides and Forssman pentasaccharide. It is strongly overexpressed in Hodgkin's disease tissue and it might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its consistently associated immunodeficiency. Recombinant Galectin-9 protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Tureci O, et al., (1997) J Biol Chem. 272(10):6416-22. Zhu C, et al., (2005) Nat Immunol. 6(12):1245-52.

ncbi gi num :

6806890

ncbi acc num :

NP_033665

ncbi gb acc num :

NM_009587.2

ncbi mol weight :

18.5kDa (168aa), confirmed by MALDI-TOF.

ncbi summary :

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: cytoplasm. Molecular Function: signal transducer activity; galactose binding; carbohydrate binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage