GOT1, 1-413aa, Human, His tag, E Coli | MyBioSource MBS203541 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

GOT1, 1-413aa, Human, His tag, E Coli

catalog :

MBS203541

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203541

products type :

Recombinant Protein

products full name :

GOT1, 1-413aa, Human, His tag, E Coli

products short name :

GOT1

products name syn :

Aspartate aminotransferase 1; Transaminase A; GIG18; Glutamic-oxaloacetic transaminase 1; Aspartate aminotransferase cytoplasmic; GIG 18; GIG18; Glutamate oxaloacetate transaminase1; Glutamic oxaloacetic transaminase 1 soluble; GOT 1; Growth inhibiting protein 18; SGOT; Transaminase

other names :

aspartate aminotransferase, cytoplasmic; Aspartate aminotransferase, cytoplasmic; aspartate aminotransferase, cytoplasmic; aspartate aminotransferase 1; aspartate transaminase 1; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; growth-inhibiting protein 18; transaminase A; glutamic-oxaloacetic transaminase 1, soluble; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

products gene name :

GOT1

other gene names :

GOT1; GOT1; cCAT; GIG18; cAspAT; ASTQTL1; cAspAT; cCAT

uniprot entry name :

AATC_HUMAN

sequence length :

413

sequence :

MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ

purity :

> 95% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22): 5293-9. Totan A., et al. (2006) Clin Chem Lab Med. 44(5):612-5.

ncbi gi num :

4504067

ncbi acc num :

NP_002070

ncbi gb acc num :

NM_002079.2

ncbi mol weight :

48.4kDa (433aa) confirmed by MALDI-TOF

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174)

ncbi summary :

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

uniprot summary :

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Protein type: Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.3; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.1; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Transferase. Chromosomal Location of Human Ortholog: 10q24.1-q25.1. Cellular Component: lysosome; cytoplasm; nerve terminal; cytosol; nucleus. Molecular Function: cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; pyridoxal phosphate binding. Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; cellular response to insulin stimulus; methionine salvage; glycerol biosynthetic process; sulfur amino acid metabolic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process. Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage