protein

S100A8, 1- 93aa, Human, Recombinant, E Coli

MBS203538

0.1 mg

375 USD

Recombinant Protein

S100A8, 1- 93aa, Human, Recombinant, E Coli

S100A8

Calgranulin-A; MRP-8; CFAG; CAGA; S100 calcium binding protein A8 60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calprotectin L1L subunit; Calprotectin; included; CGLA; Chemotactic cytokine CP-10; CP-10; Cystic fibrosisantigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; NIF; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100A8; S100A8/S100A9 complex; included; urinary stone protein band A.

protein S100-A8; Protein S100-A8; protein S100-A8; MRP-8; S100 calcium-binding protein A8 (calgranulin A); calgranulin-A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; urinary stone protein band A; S100 calcium binding protein A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band AProtein S100-A8, N-terminally processed

S100A8

S100A8; S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; CAGA; CFAG; MRP8; CFAG; MRP-8; p8

S10A8_HUMAN

93

MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

0.5 mg/ml (determined by Absorbance at 280nm)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli

Cancer

S100 calcium binding protein A8, also known as MRP8, is a member of the S100 family, EF-hand superfamily of Calcium binding proteins. S100A8 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis. Recombinant human S100A8 was expressed in E.coli and purified by using conventional chromatography techniques.

Kerkhoff C., et al. (1999) Biochim. Biophys. 1448(2):200-11. Nacken W., et al. (2003) Biochim. Biophys. 60(6):569-80.

21614544

NP_002955

NM_002964.4

10.8 Da (93aa), confirmed by MALDI-TOF

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; nucleus; cytosol. Molecular Function: arachidonic acid binding; RAGE receptor binding; protein binding; zinc ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; chemokine production; chronic inflammatory response; wound healing; cytokine production; positive regulation of peptide secretion; response to lipopolysaccharide; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; response to zinc ion; defense response to bacterium; autophagy; innate immune response; inflammatory response; defense response to fungus; acute inflammatory response; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis

0.1 mg

375 USD

0.5 mg

925