MDH2, 25-338aa, Human, His tag, E Coli | MyBioSource MBS203531 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MDH2, 25-338aa, Human, His tag, E Coli

catalog :

MBS203531

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203531

products type :

Recombinant Protein

products full name :

MDH2, 25-338aa, Human, His tag, E Coli

products short name :

MDH2

products name syn :

M-MDH; MDH; MOR1; Malate dehydrogenase 2; NAD (mitochondrial)

other names :

malate dehydrogenase, mitochondrial isoform 1; Malate dehydrogenase, mitochondrial; malate dehydrogenase, mitochondrial; malate dehydrogenase 2, NAD (mitochondrial)

products gene name :

MDH2

other gene names :

MDH2; MDH2; MDH; MOR1; M-MDH; MGC:3559

uniprot entry name :

MDHM_HUMAN

sequence length :

338

sequence :

MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25C. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 1.45 ml assay buffer. The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid. 2. Add 50 ul of recombinant MDH2 protein with various concentrations (0.015 ug, 0.03 ug, 0.06 ug) in assay buffer. 3. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.

products categories :

Signal Transduction

products description :

MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant human MDH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Thummel CS., et al. (2010) Dev Dyn. 239(3):954-64. Lee JS., et al (2002) J Basic Microbiol. 42(4):238-45.

ncbi gi num :

21735621

ncbi acc num :

NP_005909

ncbi gb acc num :

NM_005918.3

ncbi mol weight :

35.2 kDa(335aa), confirmed by MALDI-TOF

ncbi pathways :

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204); Citric Acid Cycle (TCA Cycle) Pathway (105919); Cysteine And Methionine Metabolism Pathway (104488)

ncbi summary :

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

uniprot summary :

MDH2: mitochondrial malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. May play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Mitochondrial; EC 1.1.1.37; Carbohydrate Metabolism - glyoxylate and dicarboxylate. Chromosomal Location of Human Ortholog: 7cen-q22. Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane; nucleus. Molecular Function: protein self-association; L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity. Biological Process: cellular metabolic process; malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; internal protein amino acid acetylation; gluconeogenesis

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage