protein

PKLR, 47-574aa, Human, His tag, E Coli

MBS203524

0.05 mg

375 USD

Recombinant Protein

PKLR, 47-574aa, Human, His tag, E Coli

PKLR

Pyruvate kinase isozymes R/L; PK1; PKL; PKR; PKRL; RPK

pyruvate kinase PKLR isoform 1; Pyruvate kinase PKLR; pyruvate kinase PKLR; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase isozymes L/R; pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; red cell/liver pyruvate kinase; pyruvate kinase, liver and RBC; Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase

PKLR

PKLR; PKLR; PK1; PKL; PKR; RPK; PKRL; PK1; PKL

KPYR_HUMAN

574

MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C. Assay: 1. Prepare a 1.45 ml reaction mixture into a suitable container. - Reaction mixture: 100mM Tris-HCl pH 7.5, 7.6 mM ADP, 15 mM MgCl2, 74 mM KCl, 0.2 mM Beta-NADH . 5.2 mM PEP, 0.025 units Recombinant LDHA protein . 2. Add 50ul of recombinant PKLR protein solution with various concentrations (0.5ug, 1ug) . 3. Read the decrease in A340nm in kinetic mode for 10 minutes.

Cancer

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Meza NW., et al. (2009) Mol Ther. 17(12):2000-9. Roy MF., et al. (2007) J Exp Med. 204(12):2949-61.

10835121

NP_000289

NM_000298.5

59.2 kDa (549aa), confirmed by MALDI-TOF

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); ChREBP Activates Metabolic Gene Expression Pathway (106104); Developmental Biology Pathway (477129); Disease Pathway (530764); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types. Protein type: EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Nucleotide Metabolism - purine; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytosol. Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding. Biological Process: pyruvate biosynthetic process; response to cAMP; glycolysis; response to lithium ion; positive regulation of cellular metabolic process; glucose metabolic process; pathogenesis; response to other organism; endocrine pancreas development; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; response to glucose stimulus; energy reserve metabolic process; response to hypoxia; phosphorylation; response to nutrient. Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells

0.05 mg

375 USD

0.25 mg

925