protein

ALDH3A1, 1-453aa, Human, His tag, E Coli

MBS203521

0.05 mg

375 USD

Recombinant Protein

ALDH3A1, 1-453aa, Human, His tag, E Coli

ALDH3A1

ALDHIII; ALDH3; Aldehyde dehydrogenase 3 family; memberA1 AHD 4.AHD C; AHD4; AHDC; ALDH III; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase 3 family member A1; Aldehyde dehydrogenase 3A1; Aldehyde dehydrogenase class 3; Aldehyde dehydrogenase dimeric NADP preferring; Aldehyde dehydrogenase family 3 member A1; Aldehyde dehydrogenase family 3 subfamily A1; Aldehyde dehydrogenase isozyme 3; Aldehyde dehydrogenase type III; ALDH 3; ALDH 3A1; ALDH3 A1; ALDH3A 1; MGC10406; Stomach aldehyde dehydrogenase; Tumor associated aldehyde dehydrogenase tumor ALDH.

Aldehyde dehydrogenase 3 family, memberA1; Aldehyde dehydrogenase, dimeric NADP-preferring; aldehyde dehydrogenase, dimeric NADP-preferring; aldehyde dehydrogenase isozyme 3; aldehyde dehydrogenase type III; stomach aldehyde dehydrogenase; aldehyde dehydrogenase 3 family, member A1; ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1

ALDH3A1

ALDH3A1; ALDH3A1; ALDH3; ALDHIII; ALDH3

AL3A1_HUMAN

453

MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH

> 95% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 0.5 units/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols. Assay: 1. Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 2.5ug of recombinant ALDH3A1 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes

Redox Proteins

ALDH3A1, also known as Aldehyde dehydrogenase 3 family memberA1, is involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to uV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. Recombinant ALDH3A1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Estey T., et al. (2007) Exp Eye Res. 84(1):3-12. Bogucka M, et al. (2009) Acta Pol Pharm. 66(5):477-82.

13325114

AAH04370

52.5 kDa (473aa)

2'-deoxy-alpha-D-ribose 1-phosphate Degradation Pathway 1108781!!AhR Pathway 755436!!Chemical Carcinogenesis Pathway 673221!!Chemical Carcinogenesis Pathway 673237!!Drug Metabolism - Cytochrome P450 Pathway 83032!!Drug Metabolism - Cytochrome P450 Pathway 427!!Glycolysis / Gluconeogenesis Pathway 82926!!Glycolysis / Gluconeogenesis Pathway 287!!Histidine Metabolism Pathway 82958!!Histidine Metabolism Pathway 324

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]

0.05 mg

375 USD