ACAT2, 1-397aa, Human, His tag, E Coli | MyBioSource MBS203511 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

ACAT2, 1-397aa, Human, His tag, E Coli

catalog :

MBS203511

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203511

products type :

Recombinant Protein

products full name :

ACAT2, 1-397aa, Human, His tag, E Coli

products short name :

ACAT2

products name syn :

ACTL; Acetyl-CoA acetyltransferase; cytosolic

other names :

acetyl-CoA acetyltransferase, cytosolic isoform 1; Acetyl-CoA acetyltransferase, cytosolic; acetyl-CoA acetyltransferase, cytosolic; acetoacetyl Coenzyme A thiolase; acetyl-CoA transferase-like protein; cytosolic acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase 2; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase

products gene name :

ACAT2

other gene names :

ACAT2; ACAT2; ACTL

uniprot entry name :

THIC_HUMAN

sequence length :

397

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Alger HM., et al. (2010) J Biol Chem. 285(19):14267-74. Bryleva EY., et al. (2010) Proc Natl Acad Sci u S A. 107(7):3081-6.

ncbi gi num :

148539872

ncbi acc num :

NP_005882

ncbi gb acc num :

NM_005891.2

ncbi mol weight :

45.4 kDa (433aa) confirmed by MALDI-TOF

ncbi pathways :

Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (413387); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fatty Acid Degradation Pathway (82935); Fatty Acid Degradation Pathway (296)

ncbi summary :

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

uniprot summary :

ACAT2: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq, Jul 2008]. Protein type: Carbohydrate Metabolism - butanoate; EC 2.3.1.9; Amino Acid Metabolism - tryptophan; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - pyruvate; Acetyltransferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Lipid Metabolism - fatty acid. Chromosomal Location of Human Ortholog: 6q25.3. Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus. Molecular Function: acetyl-CoA C-acetyltransferase activity. Biological Process: lipid metabolic process. Disease: Acetyl-coa Acetyltransferase-2 Deficiency

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage