product summary
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company name :
MyBioSource
product type :
protein
product name :
DDT, 1-118aa, Human, His tag, E Coli
catalog :
MBS203497
quantity :
0.1 mg
price :
285 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203497
products type :
Recombinant Protein
products full name :
DDT, 1-118aa, Human, His tag, E Coli
products short name :
DDT
products name syn :
DDCT; D-dopachtome decarboxylase; D-dopachrome tautomerase; D dopachrome decarboxylase; D dopachrome tautomerase; Phenylpyruvate tautomerase II
other names :
D-dopachrome decarboxylase; D-dopachrome decarboxylase; D-dopachrome decarboxylase; phenylpyruvate tautomerase II; D-dopachrome tautomerase; D-dopachrome tautomerase; Phenylpyruvate tautomerase II
products gene name :
DDT
other gene names :
DDT; DDT; DDCT
uniprot entry name :
DOPD_HUMAN
sequence length :
118
sequence :
MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Enzymes & Proteases
products description :
DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Arisawa K., et al. (2009) Toxicology. 255(1-2):6-14. Nishihira J., et al. (1999) Biochemistry. 38(11):3268-79.
ncbi gi num :
4503291
ncbi acc num :
NP_001346
ncbi gb acc num :
NM_001355.3
ncbi mol weight :
14.8 kDa(138aa), confirmed by MALDI-TOF
ncbi summary :
D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]
uniprot summary :
DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family. Protein type: Lyase; EC 4.1.1.84. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: cytoplasm. Molecular Function: D-dopachrome decarboxylase activity; dopachrome isomerase activity. Biological Process: melanin biosynthetic process
size1 :
0.1 mg
price1 :
285 USD
size2 :
0.5 mg
price2 :
640
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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