IMPA1, 1-277aa, Human, His tag, E Coli | MyBioSource MBS203493 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

IMPA1, 1-277aa, Human, His tag, E Coli

catalog :

MBS203493

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203493

products type :

Recombinant Protein

products full name :

IMPA1, 1-277aa, Human, His tag, E Coli

products short name :

IMPA1

products name syn :

IMPA; IMP; IMPase 1; Inositol monophosphatase 1

other names :

inositol monophosphatase 1 isoform 1; Inositol monophosphatase 1; inositol monophosphatase 1; D-galactose 1-phosphate phosphatase; IMP 1; IMPase 1; inositol-1(or 4)-monophosphatase 1; lithium-sensitive myo-inositol monophosphatase A1; inositol(myo)-1(or 4)-monophosphatase 1; D-galactose 1-phosphate phosphatase

products gene name :

IMPA1

other gene names :

IMPA1; IMPA1; IMP; IMPA; IMP 1; IMPase 1

uniprot entry name :

IMPA1_HUMAN

sequence length :

277

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

IMPA1 (inositol monophosphatase1) is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. It plays a key role in the phosphatidylinositol signaling pathway by catalyzing the hydrolysis of inositol monophosphates. This protein has been identified as the pharmacological target for lithium action in the brain. Recombinant human IMPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Schapiro MB., et al. (2002) Neurobiol Aging. 23(3):389-96. Atack JR., et al. (1992) Proc Natl Acad Sci u S A. 89(21):10031-5.

ncbi gi num :

5031789

ncbi acc num :

NP_005527

ncbi gb acc num :

NM_005536.3

ncbi mol weight :

32.3 kDa(297aa), confirmed by MALDI-TOF

ncbi pathways :

D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway (545327); D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway (138942); Inositol Phosphate Metabolism Pathway (82987); Inositol Phosphate Metabolism Pathway (730320); Inositol Phosphate Metabolism Pathway (362); Inositol Phosphate Metabolism, Ins(1,3,4,5)P4 = Ins(1,3,4)P3 = Myo-inositol Pathway (413401); Inositol Phosphate Metabolism, Ins(1,3,4,5)P4 = Ins(1,3,4)P3 = Myo-inositol Pathway (468324); Integrated Breast Cancer Pathway (219801); Metabolic Pathways (132956); Metabolism Pathway (477135)

ncbi summary :

This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Dec 2014]

uniprot summary :

IMPA1: Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol- phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1- phosphate, beta-glycerophosphate, and 2 -AMP as substrates. Belongs to the inositol monophosphatase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Phosphatase (non-protein); EC 3.1.3.94; EC 3.1.3.25; Carbohydrate Metabolism - inositol phosphate. Chromosomal Location of Human Ortholog: 8q21.13-q21.3. Cellular Component: cytoplasm; cytosol. Molecular Function: identical protein binding; protein homodimerization activity; manganese ion binding; inositol-1(or 4)-monophosphatase activity; magnesium ion binding; lithium ion binding. Biological Process: inositol phosphate metabolic process; phosphoinositide phosphorylation; inositol biosynthetic process; inositol phosphate dephosphorylation; phosphatidylinositol biosynthetic process; signal transduction; phosphate metabolic process

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage