GLuL, 1-373aa, Human, His tag, E Coli | MyBioSource MBS203490 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

GLuL, 1-373aa, Human, His tag, E Coli

catalog :

MBS203490

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203490

products type :

Recombinant Protein

products full name :

GLuL, 1-373aa, Human, His tag, E Coli

products short name :

GLuL

products name syn :

GS; GLNS; Glutamine synthetase; Glutamate-ammonia ligase; Glutamate decarboxylase

other names :

glutamine synthetase; Glutamine synthetase; glutamine synthetase; cell proliferation-inducing protein 59; glutamate decarboxylase; glutamine synthase; proliferation-inducing protein 43; glutamate-ammonia ligase; Glutamate decarboxylase (EC:4.1.1.15); Glutamate--ammonia ligase

products gene name :

GLuL

other gene names :

GLUL; GLUL; GS; GLNS; PIG43; PIG59; GLNS; GS

uniprot entry name :

GLNA_HUMAN

sequence length :

373

sequence :

MGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

Glutamine synthetase (GLuL), which is therefore able to regulate intracellular concentrations of glutamate. GLuL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLuL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLuL are associated with congenital glutamine deficiency. Recombinant GLuL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Vermeulen T., et al, (2008) Arch Biochem Biophys. 478:96-102. Liaw SH., et al. (1995) Protein Sci. 4 (11): 2358-65.

ncbi gi num :

74271837

ncbi acc num :

NP_001028216

ncbi gb acc num :

NM_001033044.3

ncbi mol weight :

44.2kDa (393aa), confirmed by MALDI-TOF

ncbi pathways :

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway (106533); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); GABA Shunt Pathway (142350); GABAergic Synapse Pathway (377263)

ncbi summary :

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

uniprot summary :

GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family. Protein type: Ligase; Energy Metabolism - nitrogen; EC 6.3.1.2; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 4.1.1.15. Chromosomal Location of Human Ortholog: 1q31. Cellular Component: protein complex; mitochondrion; rough endoplasmic reticulum; cytoplasm; perikaryon; nerve terminal; cytosol; nucleus. Molecular Function: glutamate-ammonia ligase activity; identical protein binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; manganese ion binding; magnesium ion binding; ATP binding. Biological Process: synaptic transmission; cell proliferation; glutamate catabolic process; glutamine biosynthetic process; response to glucose stimulus; positive regulation of insulin secretion; neurotransmitter uptake; amino acid biosynthetic process; cellular response to starvation; positive regulation of synaptic transmission, glutamatergic; protein homooligomerization; positive regulation of epithelial cell proliferation. Disease: Glutamine Deficiency, Congenital

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage