ADK, 22-362aa, Human, His tag, E Coli | MyBioSource MBS203486 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

ADK, 22-362aa, Human, His tag, E Coli

catalog :

MBS203486

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203486

products type :

Recombinant Protein

products full name :

ADK, 22-362aa, Human, His tag, E Coli

products short name :

ADK

products name syn :

ADK; AK; adenosine kinase; adenosine 5'-phosphotransferase

other names :

adenosine kinase isoform b; Adenosine kinase; adenosine kinase; adenosine 5'-phosphotransferase; adenosine kinase; Adenosine 5'-phosphotransferase

products gene name :

ADK

other gene names :

ADK; ADK; AK; AK

uniprot entry name :

ADK_HUMAN

sequence length :

362

sequence :

MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50mM NaCl

concentration :

0.5mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cancer

products description :

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Chakraborty, A., et al. (2002) J. Biol. Chem. 277:47451-47451. Sakowicz M., et al. (2001) Acta Biochim Pol. 48(3): 745-54.

ncbi gi num :

32484975

ncbi acc num :

NP_006712

ncbi gb acc num :

NM_006721.3

ncbi mol weight :

40.5 kDa (362aa), confirmed by MALDI-TOF

ncbi pathways :

Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Purine Salvage Pathway (106273); Adenine And Adenosine Salvage II Pathway (547496); Adenine And Adenosine Salvage II Pathway (545491); Superpathway Of Purine Nucleotide Salvage (907942)

ncbi summary :

This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

uniprot summary :

ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.20; Kinase, other; Nucleotide Metabolism - purine. Chromosomal Location of Human Ortholog: 10q22 10q11-q24. Cellular Component: nucleoplasm; cytoplasm; cytosol. Molecular Function: metal ion binding; adenosine kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding. Biological Process: nucleobase, nucleoside and nucleotide metabolic process; dATP biosynthetic process; ribonucleoside monophosphate biosynthetic process; positive regulation of T cell proliferation; circadian regulation of gene expression; adenosine metabolic process; phosphorylation; purine salvage; purine base metabolic process. Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage