protein

GSTA1, 1-222aa, Human, E Coli

MBS203481

0.1 mg

285 USD

Recombinant Protein

GSTA1, 1-222aa, Human, E Coli

GSTA1

GST2; GSTA1-1; GTH1; Glutathione S-transferase alpha 1 Glutathione S transferase A1; GST epsilon; GSTA1; GSTA1 1; HA subunit 1.

glutathione S-transferase A1; Glutathione S-transferase A1; glutathione S-transferase A1; GST HA subunit 1; GST class-alpha member 1; GST, class alpha, 1; GST-epsilon; S-(hydroxyalkyl)glutathione lyase A1; glutathione S-alkyltransferase A1; glutathione S-aryltransferase A1; glutathione S-transferase 2; glutathione S-transferase Ha subunit 1; glutathione S-transferase alpha 1; GST HA subunit 1; GST class-alpha member 1; GST-epsilon; GSTA1-1; GTH1Glutathione S-transferase A1, N-terminally processed

GSTA1

GSTA1; GSTA1; GST2; GTH1; GSTA1-1

GSTA1_HUMAN

222

MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. Assay: 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GSTA1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

Enzymes & Proteases

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Rossi D., et al. (2009) Leukemia. 23(6):1118-26. Hou L., et al. (2007) J Biol Chem. 282(32):23264-74.

22091454

NP_665683

NM_145740.3

25.6 kDa (222aa), confirmed by MALDI-TOF.

4-hydroxy-2-nonenal Detoxification Pathway (703096); 4-hydroxy-2-nonenal Detoxification Pathway (703144); AhR Pathway (755436); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660)

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

GSTA1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family. Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; EC 2.5.1.18. Chromosomal Location of Human Ortholog: 6p12.1. Cellular Component: cytosol. Molecular Function: glutathione transferase activity. Biological Process: glutathione metabolic process; epithelial cell differentiation; metabolic process; xenobiotic metabolic process

0.1 mg

285 USD

0.5 mg

640