product summary
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company name :
MyBioSource
product type :
protein
product name :
Skp, 21-161aa, E Coli, His tag, E Coli
catalog :
MBS203478
quantity :
0.1 mg
price :
285 USD
more info or order :
product information
catalog number :
MBS203478
products type :
Recombinant Protein
products full name :
Skp, 21-161aa, E Coli, His tag, E Coli
products short name :
Skp
products name syn :
Chaperone protein skp; Chaperone protein skp; periplasmic chaperone; ompH; DNA binding 17 kDa protein; ECK0177; Histone like protein HLP 1; hlpA; JW0173; Seventeen kilodalton protein
other names :
periplasmic chaperone; Chaperone protein Skp; periplasmic chaperone; DNA-binding 17 kDa protein; Histone-like protein HLP-1; Seventeen kilodalton protein
products gene name :
Skp
other gene names :
skp; skp; ECK0177; hlpA; JW0173; ompH; hlpA; ompH
uniprot entry name :
SKP_ECOLI
sequence length :
161
sequence :
MGSSHHHHHH SSGLVPRGSH MADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
concentration :
1mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Heat Shock Proteins
products description :
The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane. Recombinant E.coli Skp, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Korndorfer IP., et al. (2004) Nat Struct Mol Biol. 11(10):1015-20. Walton TA., et al. (2004) Mol Cell. 15(3):367-74.
ncbi gi num :
16128171
ncbi acc num :
NP_414720
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P0AEU7
ncbi mol weight :
17.9 kDa (162aa), confirmed by MALDI-TOF
ncbi summary :
The reported binding to DNA was an artifact due to the cationic nature of Skp. [More information is available at EcoGene: EG10455]. Skp is a periplasmic protein chaperone that binds to unfolded outer membrane proteins. [More information is available at EcoCyc: EG10455].
size1 :
0.1 mg
price1 :
285 USD
size2 :
0.5 mg
price2 :
640
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
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