product summary
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company name :
MyBioSource
product type :
protein
product name :
SORD, 1-357aa, Human, His tag, E Coli
catalog :
MBS203476
quantity :
0.1 mg
price :
285 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203476
products type :
Recombinant Protein
products full name :
SORD, 1-357aa, Human, His tag, E Coli
products short name :
SORD
products name syn :
Sorbitol dehydrogenase L iditol 2 dehydrogenase; SDH; SORD; SORD 1; SORD1
other names :
Sorbitol dehydrogenase; Sorbitol dehydrogenase; sorbitol dehydrogenase; L-iditol 2-dehydrogenase; epididymis secretory sperm binding protein Li 95n; sorbitol dehydrogenase; L-iditol 2-dehydrogenase
products gene name :
SORD
other gene names :
SORD; SORD; SORD1; HEL-S-95n
uniprot entry name :
DHSO_HUMAN
sequence length :
357
sequence :
MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli. Bioactivity: Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 25C. Assay: 1. Prepare a 1.5 ml assay buffer. The final concentrations are 78 mM Triethanolamine, 183 mM D-Fructose, 0.2 mM beta-nicotinamide dinucleotide and 0.003 % (w/v) bovine serum albumin. 2. Add 50 ul of recombinant SORD protein in various concentrations (1 ug, 2 ug, 5 ug) in assay buffer. 3. Mix by inversion and record the decrease at A340nm for 5 minutes.
products categories :
Metabolism
products description :
SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357 amino acid member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Baker MA., et al. (2010) Proteomics. 10(3):482-95. Sorger D., et al. (2009) Nucl Med Biol. 36(1):17-27.
ncbi gi num :
19263809
ncbi acc num :
AAH25295
ncbi mol weight :
40.4 kDa (377aa) confirmed by MALDI-TOF
ncbi pathways :
Fructose And Mannose Metabolism Pathway 82930!!Fructose And Mannose Metabolism Pathway 291!!Glucuronate Pathway (uronate Pathway) 997799!!Glucuronate Pathway (uronate Pathway) 468207!!Metabolic Pathways 132956!!Pentose And Glucuronate Interconversions Pathway 82929!!Pentose And Glucuronate Interconversions Pathway 290!!Polyol Pathway 198815!!Sorbitol Degradation I Pathway 142439
ncbi summary :
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
size :
0.1 mg
price :
285 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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