protein

NuDT5, 1-219aa, Human, His tag, E Coli

MBS203467

0.1 mg

310 USD

Recombinant Protein

NuDT5, 1-219aa, Human, His tag, E Coli

NuDT5

hYSAH1; YSA1; YSA1H; ADP-sugar pyrophosphatase; HSPC115; Nudix motif 5; NuDT-5; NuDT 5

ADP-sugar pyrophosphatase; ADP-sugar pyrophosphatase; ADP-sugar pyrophosphatase; 8-oxo-dGDP phosphatase; nudix (nucleoside diphosphate linked moiety X)-type motif 5; 8-oxo-dGDP phosphatase (EC:3.6.1.58); Nucleoside diphosphate-linked moiety X motif 5; Nudix motif 5; YSA1H

NuDT5

NUDT5; NUDT5; YSA1; YSA1H; YSAH1; hYSAH1; Nudix motif 5

NUDT5_HUMAN

219

MGSSHHHHHH SSGLVPRGSH MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF

> 85 % by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Enzymes & Proteases

NuDT5 is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NuDT5 hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium, and also hydrolyzes other nucleotide sugars with low activity such as ADP-glucose and diadenosine diphosphate. As a nudix hydrolase, NuDT5 contains a central nudix motif and functions to eliminate toxic nucleotide metabolites from the cell while maintaining the levels of signaling nucleotides. NuDT5 is widely expressed but is most abundant in liver as a homodimer. Recombinant human NuDT5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Yu HN., et al. (2007) Biochem Biophys Res Commun. 354(3):764-8.

37594464

NP_054861

NM_014142.2

26.5 kDa (239aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will be appear higher)

Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Phosphate Bond Hydrolysis By NUDT Proteins Pathway (730342); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307)

This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013]

NUDT5: Hydrolyzes with similar activities ADP-ribose ADP- mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. Belongs to the Nudix hydrolase family. Protein type: Phosphatase; EC 3.6.1.13; EC 3.6.1.58; Nucleotide Metabolism - purine. Chromosomal Location of Human Ortholog: 10p14. Cellular Component: intracellular; nucleus; cytosol. Molecular Function: snoRNA binding; m7G(5 )pppN diphosphatase activity; ADP-ribose diphosphatase activity; nucleoside-diphosphatase activity; magnesium ion binding; ADP-sugar diphosphatase activity. Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; D-ribose catabolic process; ribonucleoside diphosphate catabolic process

0.1 mg

310 USD

0.5 mg

720