PGAM1, 1-254aa, Human, His tag, E Coli | MyBioSource MBS203464 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

PGAM1, 1-254aa, Human, His tag, E Coli

catalog :

MBS203464

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203464

products type :

Recombinant Protein

products full name :

PGAM1, 1-254aa, Human, His tag, E Coli

products short name :

PGAM1

products name syn :

Phosphoglycerate mutase isozyme B; PGAM-B; PGAMA; Phosphoglycerate mutase 1

other names :

phosphoglycerate mutase 1; Phosphoglycerate mutase 1; phosphoglycerate mutase 1; BPG-dependent PGAM 1; epididymis secretory protein Li 35; phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase isozyme B; phosphoglycerate mutase 1 (brain); BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B

products gene name :

PGAM1

other gene names :

PGAM1; PGAM1; PGAMA; PGAM-B; HEL-S-35; PGAMA; PGAM-B

uniprot entry name :

PGAM1_HUMAN

sequence length :

254

sequence :

MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK

purity :

> 90 % by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cancer

products description :

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Sakoda S., et al. (1988) J Biol Chem. 263(32):16899-905. Junien C., et al. (1982) Ann Genet. 25(1):25-7.

ncbi gi num :

4505753

ncbi acc num :

NP_002620

ncbi gb acc num :

NM_002629.2

ncbi mol weight :

30.9 kDa (274aa), confirmed by MALDI-TOF

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

ncbi summary :

Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]

uniprot summary :

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. Protein type: EC 3.1.3.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; EC 5.4.2.4; Phosphatase (non-protein); EC 5.4.2.11. Chromosomal Location of Human Ortholog: 10q25.3. Cellular Component: membrane; cytoplasm; cytosol. Molecular Function: protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding. Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage