protein

ECHS1, 28-290aa, Human, His tag, E Coli

MBS203459

0.1 mg

310 USD

Recombinant Protein

ECHS1, 28-290aa, Human, His tag, E Coli

ECHS1

Enoyl Coenzyme A hydratase; short chain 1 mitochondrial; SCEH

Enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase, mitochondrial; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; enoyl-CoA hydratase 1; short-chain enoyl-CoA hydratase; enoyl CoA hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH

ECHS1

ECHS1; ECHS1; SCEH; SCEH

ECHM_HUMAN

290

MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Metabolism

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Kanazawa M., et al. (1993) Enzyme Protein. 47:9-13. Stern J R., et al. (1956) J Biol Chem. 218:985-1002.

14286220

AAH08906

30.6 kDa (284aa), confirmed by MALDI-TOF

Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway (106130); Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway (106127); Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway (106129); Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway (106126); Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway (106128); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Fatty Acid Beta Oxidation Pathway (198865)

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]

ECHS1: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Belongs to the enoyl-CoA hydratase/isomerase family. Protein type: EC 4.2.1.17; Lipid Metabolism - fatty acid elongation in mitochondria; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Mitochondrial; Lyase; Secondary Metabolites Metabolism - limonene and pinene degradation; Carbohydrate Metabolism - propanoate; Lipid Metabolism - fatty acid. Chromosomal Location of Human Ortholog: 10q26.2-q26.3. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: protein binding; enoyl-CoA hydratase activity. Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process. Disease: Mitochondrial Short-chain Enoyl-coa Hydratase 1 Deficiency

0.1 mg

310 USD

0.5 mg

720