protein

FKBP3, 1-224aa, Human, E Coli

MBS203458

0.1 mg

285 USD

Recombinant Protein

FKBP3, 1-224aa, Human, E Coli

FKBP3

FKBP25; PPIase FKBP3; Rotamase; FK506 binding protein 3 FKBP 25; FKBP 3; PPIase; Rotamase.

peptidyl-prolyl cis-trans isomerase FKBP3; Peptidyl-prolyl cis-trans isomerase FKBP3; peptidyl-prolyl cis-trans isomerase FKBP3; 25 kDa FK506-binding protein; 25 kDa FKBP; FK506-binding protein 25, T-cell; FK506-binding protein 3 (25kD); PPIase FKBP3; immunophilin FKBP25; rapamycin binding protein; rapamycin-selective 25 kDa immunophilin; rotamase; FK506 binding protein 3, 25kDa; 25 kDa FK506-binding protein; 25 kDa FKBP; FKBP-25; FK506-binding protein 3; FKBP-3; Immunophilin FKBP25; Rapamycin-selective 25 kDa immunophilin; Rotamase

FKBP3

FKBP3; FKBP3; FKBP-3; FKBP25; PPIase; FKBP-25; FKBP25; PPIase FKBP3; 25 kDa FKBP; FKBP-25; FKBP-3

FKBP3_HUMAN

224

MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity is > 490 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant FKBP3 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

Heat Shock Proteins

FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E.coli and purified by using conventional chromatography techniques.

Yang WM., et al. (2001) EMBO J. 20(17):4814-25. Jin YJ., et al. (1993) Proc Natl Acad Sci u S A. 90(16):7769-73.

4503727

NP_002004

NM_002013.3

25.1 kDa (224aa), confirmed by MALDI-TOF.

Integrated Pancreatic Cancer Pathway 711360!!Signaling Events Mediated By HDAC Class I Pathway 137997

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. [provided by RefSeq, Sep 2008]

0.1 mg

285 USD